基于ISSR标记分析浙江省樱花种质资源的遗传多样性  

Genetic Diversities of Cerasus spp. from Zhejiang Province Based on ISSR Markers

作　　者：蒋冬月[1,2] 沈鑫 吴帆[1] 李因刚[1] 石从广[1] 杨少宗[1] 柳新红[1] JIANG Dong-yue;SHEN Xin;WU Fan;LI Yin-gang;SHI Cong-guang;YANG Shao-zong;LIU Xin-hong(Zhejiang Academy of Forestry,Hangzhou 310023,China;Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China)

机构地区：[1]浙江省林业科学研究院,浙江杭州310023 [2]中国科学院植物研究所,北京100093

出　　处：《浙江林业科技》2018年第4期1-7,共7页Journal of Zhejiang Forestry Science and Technology

基　　金：浙江省樱亚属遗传多样性研究(2017F30017);国产樱花新品系无性快繁技术体系研发(2016F50024);浙江省十三五林木新品种选育项目(2016C02056-12)

摘　　要：为探究浙江省樱花种质资源的亲缘关系和遗传多样性,运用ISSR分子标记从100条引物中筛选出17条多态性好的引物对95份樱花种质资源进行分析。结果表明,17条引物共扩增出110条清晰条带,其中101条具有多态性,平均多态性条带比率为91.78%。95份资源间的遗传相似系数为0.38～0.90,平均0.61;遗传距离为0.10～1.38,平均0.50。根据UPGMA聚类分析,在遗传相似系数为0.577 5时,95份种质资源可分为四大类,分别包含了23份、42份、25份和5份材料。研究结果表明浙江省樱花种质资源具有较为丰富的遗传多样性。Collections of 95 germplasm resources of Cerasus spp.were carried out in May and June of 2016 from 14 counties of Zhejiang province.Experiments were implemented for their genetic diversities using 17 ISSR(inter simple sequence repeat)markers from 100 ones.The results showed that 110 bands were amplified by 17 primers,in which 101 bands were polymorphic with average percentage of ISSR polymorphism of 91.78%.The genetic similarity coefficients of tested resources ranged from 0.38 to 0.90,with an average of 0.61.The genetic distances ranged from 0.10 to 1.38,with an average of 0.5.UPGMA(unweighted pair-group method with arithmetic means)analysis demonstrated that 95 germplasm resources could be divided into four categories when genetic similarity coefficient was 0.5775.The results indicated that there were rich genetic diversities in Cerasus spp.from Zhejiang.

关 键 词：樱花 种质资源 ISSR 遗传多样性 浙江 

分 类 号：S685.99[农业科学—观赏园艺]