松材线虫果胶酶基因Bxpel1的克隆及其生物信息学分析  

Cloning and Bioinformatics Analysis of Bxpel1 Gene from Bursaphelenchus xylophilus

作　　者：邱秀文[1,2,3] 周桂香[1,2,3] 王慧娟[3] 杨丽丽[3] QIU Xiu-wen;ZHOU Gui-xiang;WANG Hui-juan;YANG Li-li(Poyang Lake Eco-economy Research Center,Jiujiang University,Jiujiang 332005,China;Jiujiang Key Laboratory of Basin Management and Ecological Protection,Jiujiang University,Jiujiang 332005,China;School of Chemistry and Environmental Engineering,Jiujiang University,Jiujiang 332005,China)

机构地区：[1]九江学院鄱阳湖生态经济研究中心,江西九江332005 [2]九江学院九江市流域管理与生态保护重点实验室,江西九江332005 [3]九江学院化学与环境工程学院,江西九江332005

出　　处：《浙江林业科技》2018年第4期8-14,共7页Journal of Zhejiang Forestry Science and Technology

基　　金：国家自然科学基金项目(31660205);江西省自然科学基金项目(20161BAB214152;20151BAB213018)

摘　　要：以松材线虫Bursaphelenchus xylophilus转录本为模板,通过RT-PCR技术克隆果胶酶Bxpel1基因。结果表明,松材线虫果胶酶Bxpel1基因序列全长为795bp,GC含量为50.44%,编码252个氨基酸。通过Blast比对克隆的Bxpel1基因与已知序列(Gen-Bank ID:AB232908.1)的同源性达到98%。系统进化分析表明,Bxpel1基因编码产物的氨基酸序列与拟松材线虫Bursaphelenchus mucronatus,燕麦真滑刃线虫Aphelenchus avenae的Bxpel1蛋白序列具有高度同源性。Bxpel1基因编码的产物的0～25序列有可能是跨膜结构域,而其他序列均位于膜外,其二级结构主要是以无规则卷曲和β-折叠为主,信号肽的剪切位点位于第17至第18位氨基酸之间,主要在细胞外发挥生物学作用。Bxpel1基因的克隆及其生物信息学分析对进一步研究Bxpel1基因在松材线虫致病过程中的功能具有重要意义。Bxpel1 gene sequence from Bursaphelenchus xylophilus cDNA as a template was amplified and cloned by RT-PCR.Bxpel1 gene(795bp)was successfully cloned with GC content of 50.44%and encode of 252 amino acids.The cloned sequence had 98%similarity to the pel1 gene previously published in the GenBank(ID:AB232908.1).Phylogenetic analysis showed that Bxpel1 had high homologies with that from B.mucronatus and Aphelenchus avenae.Bxpel1 gene encode from 0 to 25 sequences may be transmembrane structure,while the other sequences outside membrane.The secondary and tertiary structures were abundant in random coils and beta helix.The signal peptide cleavage sites locate between No.17 to No.18 amino acid and performs biological effects at extracellular.

关 键 词：松材线虫 Bxpel1基因 克隆 生物信息学 

分 类 号：S763[农业科学—森林保护学]