二氢杨梅素对高脂喂养ApoE^(-/-)小鼠体内胆固醇逆向转运的影响  

Effect of dihydromyricetin on reverse cholesterol transport in high-fat feeding ApoE^(-/-) mice

作　　者：朱景平 卫欣妤 许晓乐 ZHU Jing-ping;WEI Xin-yu;XU Xiao-le(Dept of Clinical Pharmacy,the First Affiliated Hospital of Nanyang Medical College,Nanyang Henan 473000,China;Dept of Pharmacology,Pharmacy College,Nantong University,Nantong Jiangsu 226001,China)

机构地区：[1]南阳医学高等专科学校第一附属医院临床药学科,河南南阳473000 [2]南通大学药学院药理系,江苏南通226001

出　　处：《中国药理学通报》2018年第11期1610-1616,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81200079);江苏省"六大人才高峰"第十二批高层次人才培养项目(No SWYY-022);南通市科技计划项目(No MS12015045)

摘　　要：目的探讨二氢杨梅素(dihydromyricetin,DMY)对高脂喂养ApoE^(-/-)小鼠体内胆固醇逆向转运的影响。方法 6周龄♂ApoE^(-/-)小鼠分为模型组、DMY(250、500 mg·kg^(-1))组,野生型(WT)C57BL/6J小鼠为阴性对照组。各组高脂饮食8周后,腹腔巨噬细胞油红O染色,测定细胞内胆固醇含量,肝脏称重,肝脏组织油红O染色,检测肝脏胆固醇和甘油三酯含量,检测巨噬细胞、肝脏和小肠中胆固醇逆向转运相关蛋白的表达。结果与模型组相比,DMY明显减少腹腔巨噬细胞脂质的沉积和胆固醇含量,增强巨噬细胞胆固醇逆向转运蛋白ABCA1和ABCG1的表达。与模型组相比,DMY明显减轻肝脏脂肪变性,即减轻肝脏脂质蓄积、胆固醇和甘油三酯含量。与模型组相比,DMY明显增加肝脏ABCG5、ABCG8、CYP7A1的mRNA和蛋白表达,DMY明显增加小肠ABCG5和ABCG8的蛋白表达。结论 DMY能促进高脂喂养ApoE^(-/-)小鼠体内胆固醇逆向转运。Aim To study the effect of dihydromyricetin(DMY)on reverse cholesterol transport(RCT)regulation in ApoE-/-mouse model.Methods Six-week-old male ApoE-/-mice of C57BL/6J background and age-matched wild-type(WT)C57BL/6J controls were fed a high fat diet(HFD)for eight weeks.ApoE-/-mice were dosed daily via intragastric gavage with 250 or 500 mg·kg^-1 DMY or administered vehicle alone as a model group.After eight weeks,mouse peritoneal macrophages were collected.Quantifications of hepatic lipid content,gene expressions were performed on peritoneal macrophage,liver and intestine samples.Results The data showed that compared with model group,DMY suppressed HFD-induced macrophage foam cell formation and hepatic steatosis in ApoE-/-mice,and increased macrophage ABCA1 and ABCG1 protein expression.In addition,DMY reduced hepatic lipid content.DMY increased ABCG5,ABCG8 and CYP7A1 mRNA and protein expression in the liver.DMY also increased ABCG5 and ABCG8 protein expression in the intestine.Conclusion DMY could promote RCT in HFD-fed ApoE-/-mice.

关 键 词：二氢杨梅素 胆固醇逆向转运 APOE-/-小鼠 巨噬细胞 肝脏 小肠 

分 类 号：R284.1[医药卫生—中药学] R322.45[医药卫生—中医学]