低剂量三苯基锡暴露对鲤GH/IGF-I轴的毒性效应  

The toxicity effects of low dose triphenyltin(TPT) on GH/IGF-Iaxis in Cyprinus carpio

作　　者：刘伟[1,2] 钟利桥[2,3] 解华晓[1,2] 吴路银 姚凡 倪朝辉 LIU Wei;ZHONG Li-qiao;XIE Hua-xiao;WU Lu-yin;YAO Fan;NI Zhao-hui(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Fishery Eco-environmental Monitoring Center in the Upper and Middle Reaches of the Yangtze River,Ministry of Agriculture; Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;Freshwater Fisheries Research Center,Chinese Academy of Fisheries Science,Wuxi 214081,Jiangsu,China;College of Fisheries,Huazhong Agricultural University,Wuhan,430070,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院长江水产研究所,农业部长江中上游渔业生态环境监测中心,武汉430223 [3]中国水产科学研究院淡水渔业研究中心,江苏无锡214081 [4]华中农业大学水产学院,武汉430070

出　　处：《淡水渔业》2018年第6期3-8,共6页Freshwater Fisheries

基　　金：国家自然科学基金青年科学基金项目(21507162);中国水产科学研究院淡水渔业研究中心公益性科研院所基本科研业务费专项资金项目(2015JBFM35);公益性行业(农业)科研专项(201503108)。

摘　　要：为探究环境内分泌物对鱼类生长的毒性影响及作用机制,以鲤(Cyprinus carpio)为实验动物,利用三苯基氯化锡(TPT)为目标毒物,设定对照组和0. 024、0. 24、2. 4μg/L三个TPT暴露组,不同时间(7、21、48 d)暴露取样,通过检测血浆生长激素(GH)和胰岛素样生长因子(IGF-I)水平及肝脏生长激素受体(GHR)、IGF-I基因和脑垂体GH基因的表达量,探讨三苯基锡暴露对鲤GH/IGF-I轴毒性效应的影响及其机制。结果表明:暴露7 d时,高浓度的TPT(0. 24、2. 4μg/L)促进垂体GH的合成与分泌,而IGF-I的合成与分泌及肝脏IGF-I基因的表达受到显著抑制;暴露21、48 d时,血浆GH、IGF-I含量显著下降,脑垂体GH基因及肝脏GHR、IGF-I基因的表达均受到显著性抑制且呈剂量效应关系。研究结果表明,鲤血浆GH、IGF-I含量及肝脏GHR、IGF-I基因和垂体GH基因的表达量对不同浓度的TPT反应敏感,可以作为监测环境污染物对鱼类GH/IGF-I轴毒性影响的指示因子。Cyprinus carpio were exposed to 0,0.024,0.24,2.4μg/L Triphenyltin(TPT)for 7,21,48 days to countersign the effects of environmental endocrine disruptors on development of fish and explore the mechanisms.Hematological parameters and the expression of genes related GH/IGF-I axis were analyzed to probe into the toxic effects of TPT on the growth and GH/IGF-I axis of C.Carpio.The results showed that after 7 days exposure,high concentration TPT(0.24 and 2.4μg/L)irritated the synthesis and secretion of pituitary GH,while the synthesis and secretion of IGF-I and the expression of hepatic IGF-I were significantly inhibited.Plasma GH and IGF-I contents were significantly decreased,and the ex pression of pituitary GH and GHR and IGF-I in liver were significantly inhibited with dose-effect relationship after TPT(0.24 and 2.4μg/L)exposed for 21days and 48days.This study indicated the content of plasma GH and IGF-I as well as the expression of pituitary GH and GHR,IGF-I in liver was sensitive to different concentrations of TPT reaction.So these could be used as monitors to detect the toxicity effects of environmental pollutants on the growth and GH/IGF-I axis in fisheries.

关 键 词：三苯基锡 鲤(Cyprinus carpio) GH/IGF-I轴 毒性效应 

分 类 号：X503.225[环境科学与工程—环境工程]