卡瓦叶子的化学成分和细胞毒活性研究  

Investigation on the Chemical Constituents and Cytotoxicity from the Leaves of Kava

作　　者：杨建香[1] YANG Jian-xiang(Editorial Department,Guilin Normal College,Guilin 541199,Guangxi,China)

机构地区：[1]桂林师范高等专科学校学报编辑部,广西桂林541199

出　　处：《湖北农业科学》2018年第20期93-97,共5页Hubei Agricultural Sciences

基　　金：桂北特色药资源研究中心科研项目(KYA201702)

摘　　要：选取卡瓦(Piper methysticum Forst. f.)叶子为材料,通过多次硅胶柱层析、凝胶柱层析等方法对各个部位进行分离纯化,然后借助于核磁共振氢谱、碳谱、质谱等方法进行结构鉴定,最终从卡瓦叶子中分离出6种化合物,分别为desmethoxyyangonin、yangonin、11-methoxyyangonin、11-methoxy-5,6-dihydroyangonin、methysticin、dihydromethysticin。采用MTT法对6种化合物进行药理活性测试,结果表明,Yangonin和11-methoxyyangonin对细胞A549(非小细胞肺癌)和细胞NCI-H460(人体肺癌细胞)有非常强的细胞毒活性,11-methoxy-5,6-dihydroyangonin、Methysticin、Dihydromethysticin对细胞A549和细胞NCI-H460则没有明显的细胞毒性。Taking leaves of Kava(Piper methysticum Forst.f.)as materials,six compounds were obtained from the EtOAc extract by a combination of column chromatography of silica gel,Sephadex LH-20 many times.Their structures were determined on the basis of 1HNMR,13C NMR,2DNMR,UV,IR,and ESIMS spectra data and were identified as Desmethoxyyangonin,yangonin,11-methoxyyangonin,11-methoxy-5,6-dihydroyangonin,methysticin,dihydromethysticin.The cytotoxicity of six compoundswas tested by MTT assay,the result showed that yangonin and 11-methoxyyangonin were very strong cytotoxicity against A549 and NCI-H460 cells,while,11-methoxy-5,6-dihydroyangonin,methysticin and dihydromethysticin were very weak cytotoxicity against A549 and NCI-H460 cells.

关 键 词：卡瓦(PipermethysticumForst.f.)叶子 化学成分 细胞毒活性 

分 类 号：O629[理学—有机化学] R962[理学—化学]