20(S/R)-人参皂苷Rg_3的制备及调节Th1/Th2免疫失衡活性  

Preparation of 20(S/R)-Ginsenoside Rg_3 and Their Effects on Regulation of Th1/Th2 Imbalance

作　　者：刘迎[1] 陈妍心 吴谦 黎鹏 李绪文[1] 时晓磊 金永日[1] LIU Ying;CHEN Yanxin;WU Qian;LI Peng;LI Xuwen;SHI Xiaolei;JIN Yongri(College of Chemistry,Jilin University,Changchun 130012,China;College of Food Science and Engineering,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学化学学院,长春130012 [2]吉林大学食品科学与工程学院,长春130062

出　　处：《高等学校化学学报》2018年第11期2419-2424,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:31201295)资助~~

摘　　要：采用醋酸溶液作为提取溶剂,使西洋参叶中的二醇组人参皂苷在提取过程中发生降解,从而直接获得20(S)-人参皂苷Rg_3和20(R)-人参皂苷Rg_3,并对其调节Th1/Th2免疫失衡活性进行了研究.正交实验结果表明,当醋酸浓度为50%(体积分数),提取温度为80℃,提取时间为1 h时,20(S)-人参皂苷Rg_3和20(R)-人参皂苷Rg_3的转化率最高,分别为12. 30%和14. 80%.将20(S)-人参皂苷Rg_3和20(R)-人参皂苷Rg_3处理后的朗格汉斯状树突细胞(LDCs)分别作用于小鼠抗原诱导的Th1/Th2免疫失衡模型,发现细胞上层清液中IL-4的水平均显著降低,说明20(S)-人参皂苷Rg_3和20(R)-人参皂苷Rg_3对小鼠Th1/Th2免疫失衡具有调节作用.本文不仅建立了一种制备20(S)-人参皂苷Rg_3和20(R)-人参皂苷Rg_3的新方法,也为人参皂苷Rg_3在免疫系统疾病中的应用提供了新的科学依据.20(S)-Ginsenoside Rg 3 and 20(R)-ginsenoside Rg 3 were prepared by degradation and extraction methods from leaves of Panax quinquefolium L.The regulation effects of 20(S)-Rg 3 and 20(R)-Rg 3 on imbalance of Th1/Th2 cell were studied in vivo.As the orthogonal array optimization results shown,when the concentration of acetic acid was 50%,temperature of 80℃and extraction time of 1 h,the conversion rates of 20(S)-Rg 3 and 20(R)-Rg 3 were highest at 12.30%and 14.80%,respectively.Then the 20(S/R)-Rg 3 were purified by chromatographic method.According to the experimental results of activity,20(S/R)-Rg 3 inhibited the Th2 cells development by down-regulation of IL-4 production.It was associated with the CD40 and TIM-4 expression of LDCs.This work reports a new method to prepare ginsenoside Rg 3 and draw a novel biological mechanism of ginsenoside Rg 3,effects on imbalance of Th1/Th2.

关 键 词：降解 提取 20(S/R)-人参皂苷Rg3 Th1/Th2免疫失衡 

分 类 号：O629[理学—有机化学]