鞘内注射HDAC抑制剂增加持续性术后痛大鼠脊髓TNF-α的含量  

INTRATHECAL INJECTION OF HDAC INHIBITOR INCREASES THE CONCENTRATION OF TNF-α IN THE SPINAL CORD OF RATS WITH PERSISTENT POSTOPERATIVE PAIN

作　　者：胡兴国[1] 宋立娟 张励才 HU Xing-Guo;SONG Li-Juan;ZHANG Li-Cai(Department of anesthesiology,Taoyuan County People's Hospital,Hunan,Taoyuan 415700,China;Department of Anesthesiology,Binzhou Medical University Hospital,Binzhou 256603,China;Jiangsu Province Key Laboratory of Anesthesiology&Jiangsu Province Laboratory of Anesthetic and Analgesia Application Technology,Xuzhou Medicine University,Xuzhou 221004,China)

机构地区：[1]湖南省桃源县人民医院麻醉科,桃源415700 [2]山东省滨州医学院附属医院麻醉科,滨州256600 [3]徐州医科大学江苏省麻醉学重点实验室&江苏省麻醉与镇痛应用技术重点实验室,徐州221004

出　　处：《中国疼痛医学杂志》2018年第10期733-737,共5页Chinese Journal of Pain Medicine

基　　金：江苏省教育厅省级高校重点实验室开放课题(KJS1409);湖南省高层次卫生人才225工程培养对象项目

摘　　要：目的:研究鞘内注射组蛋白去乙酰化酶(histone deacetylase, HDAC)抑制剂对皮肤/肌肉切口和牵拉(skin/muscle incision and retraction, SMIR)持续性术后痛大鼠脊髓肿瘤坏死因子α(tumor necrosis factorα, TNF-α)含量的影响。方法:清洁级健康雄性SD大鼠64只,采用随机数字表法随机分为8组(n=8/组),假手术+人工脑脊液组(Ⅰ组),假手术+二甲基亚砜组(Ⅱ组),假手术+辛二酰苯胺异羟肟酸(suberoylanilide hydroxamic acid, SAHA)组(Ⅲ组),假手术+曲古菌素A (Trichostatin A,TSA)组(Ⅳ组),SMIR+人工脑脊液组(Ⅴ组),SMIR+二甲基亚砜组(Ⅵ组),SMIR+SAHA组(Ⅶ组)和SMIR+TSA组(Ⅷ组)。结果:与Ⅰ组和T0比较,Ⅴ-Ⅷ组在T2-5时MWT明显下降(P <0.05),T4时脊髓TNF-α含量明显升高(P <0.05);与Ⅴ组比较,Ⅶ-Ⅷ组在T3-5时MWT明显升高(P <0.05),T4时脊髓TNF-α含量明显下降(P <0.05)。结论:鞘内给予HDAC抑制剂对持续性术后痛大鼠具有镇痛作用,持续性术后痛时脊髓TNF-α含量增加可能与组蛋白乙酰化异常调节有关。Objective:To study the effects of intrathecal treatment with histone deacetylase(HDAC)inhibitors on the concentration of tumor necrosis factor-α(TNF-α)in the spinal cord in a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction(SMIR).Methods:Sixty-four pathogen-free healthy male SD rats were randomly divided into 8 groups(n=8 each group):Sham+artificial cerebrospinal fluid(ACSF)group(group),Sham+dimethyl sulfoxide(DMSO)group(group),Sham+suberoylanilide hydroxamic acid(SAHA)group(group),Sham+Trichostatin A(TSA)group(group),SMIR+ACSF group(group),SMIR+DMSO group(group),SMIR+SAHA group(groupⅦ)and SMIR+TSA group(groupⅧ).Results:Compared with groupⅠand T0,the MWT was decreased at T2-5(P<0.05),and the concentration of TNF-αin the spinal cord was increased at T4 in group-(P<0.05).Compared with groupⅤ,the MWT was increased at T3-5(P<0.05),and the concentration of TNF-αin the spinal cord was decreased at T4 in groupⅦ-Ⅷ(P<0.05).Conclusion:Intrathecal treatment with HDAC inhibitor has analgesic effect on the rats with persistent postoperative pain.The increase of TNF-αin spinal cord may be related with histone acetylation abnormal regulation in a rat model of persistent postoperative pain evoked by SMIR.

关 键 词：组蛋白去乙酰化酶 乙酰化作用 肿瘤坏死因子Α 术后痛 脊髓 

分 类 号：R614[医药卫生—麻醉学]