猪红斑丹毒丝菌云南YNKMYZS-2016株的分离鉴定  

IDENTIFICATION AND ISOLATION OF ERYSIPELOTHRIX RHUSIOPATHIAE YNKMYZS-2016, IN YUNNAN

作　　者：郑碧妞 陶松[2] 岩锐 王建聂 陈建华 字品文 姚俊[7] 王生奎[2] ZHENG Bi-niu;TAO Song;YAN Rui;WANG Jian-nie;CHEN Jian-hua;ZI Pin-wen;YAO-Jun;WANG Sheng-kui(Bureau of Agricultural Science and Tehnology in Lushui City,Nujiang of the Lisu Autonomous Prefecture,Lushui 673199,China;College of Animal Science and Technology,Yunnan Agricultural University,Kunning 650201,China;Bureau of Agricultural Science and Technology in Puer City,Menglian Daizu Lahuzu Wazu Autonomous County,Menglian 665800,China;Liujing Township Comprehensive Agricultural Service Station,Wenshan City,Yunnan Province,Wenshan 663012,China;Burean of Agricultural Science and Technology in Lijiang City,Ninglang Yizu Autonomous County,Ninglang,673500,China;Agricultural Service Center in Guoqiao Town,Yunlong County,Dali Bai Autonomous Prefecture,Yunlong 672708,China;Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory,Yunnan Animal Science and Veterinary Institute,Kunming 650224,China)

机构地区：[1]云南省怒江傈僳族自治州泸水市农业和科学技术局,泸水673199 [2]云南农业大学动物科学技术学院,昆明650201 [3]云南省普洱市孟连傣族拉祜族佤族自治县农业和科学技术局,孟连665800 [4]云南省文山市柳井彝族乡农业综合服务中心,文山663012 [5]云南省丽江市宁蒗彝族自治县农业和科学技术局,宁蒗673500 [6]云南省大理白族自治州云龙县果桥镇农业综合服务中心,云龙672708 [7]云南省畜牧兽医科学院云南省热带亚热带动物病毒病重点实验室,昆明650224

出　　处：《中国动物传染病学报》2018年第5期86-93,共8页Chinese Journal of Animal Infectious Diseases

基　　金：云南省省本级财政专项(20161105560334)

摘　　要：2016年10月,云南省昆明市某规模化猪场饲养的妊娠后期母猪频繁发生急性死亡,为了尽快确诊,采集病死猪及胎儿内脏组织病料进行PCR及real-time PCR检测,同时进行病毒及细菌分离培养鉴定。结果显示,从病死猪肺脏组织分离获得1株猪红斑丹毒丝菌,其16S rRNA序列与NCBI GenBank中的猪红斑丹毒丝菌参考菌株Fujisawa(登录号:NZ_015601.1)、GXBY-1(登录号:NZ_CP014861.1)、KG-BB1(登录号:AB055909.1)、KG-BB2(登录号:AB055910.1)、SY1027(登录号:NZ_021354.1)、WH13013(登录号:NZ_CP017116.1)的16SrRNA序列相似性达96.3%以上。该分离菌株对青霉素、红霉素、阿莫西林、阿莫西林/克拉维酸、原始霉素、苯唑西林、泰落星、头孢噻吩、头孢哌酮、呋喃妥因敏感,对林可霉素、多粘菌素E、复方新诺明、链霉素、磺胺甲恶唑、大观霉素、氟甲喹、卡那霉素、庆大霉素、衣氟沙星、奥索利酸、安普霉素、夫西地酸、氯霉素、四环素、利福平、多西环素、甲硝唑耐药。本研究结果为该疫情的预防治疗提供了科学参考。In October,2016,acute deaths frequently occurred among pregnant sows on a large scale pig farm in Kunming,Yunnan province.Tissue samples from dead sows and aborted fetuses were collected for isolation of CSFV,PRRSV,PPV,PRV,JEV,Chlamydia psittaci,Toxoplasma gondii,Pasteurella multocida and detection by conventional PCR or real time PCR assays.As a result,one strain of Erysipelothrix rhusiopathiae designated as ynkmyzs-2016 was isolated from dead sow lungs but none of CSFV,PRRSV,PPV,PRV,JEV,Chlamydia psittaci,Toxoplasma gondii and Pasteurella multocida were detected.The 16S rRNA sequence of this strain had 96.3%identity to that of the reference strains Fujisawa(Accession No.NZ_015601.1),GXBY-1(Accession No.NZ_CP014861.1),KG-BB1(Accession No.AB055909.1),KG-BB2(Accession No.AB055910.1v,SY1027(Accession No.NZ_021354.1)and WH13013(Accession No.NZ_CP017116.1)The drug test showed that this strain was susceptible to penicillin,erythromycin,amoxicillin,amoxicillin/clavulanic acid,mycophenolate,oxacillin,sulbactam,cefoxitin and cefoperazone and furantoin but resistant to lincomycin,polymyxin E,compound sulfamethoxazole,streptomycin,sulfamethoxazole,spectinomycin,flumequine,kanamycin,gentamicin,levofloxacin clothing,oxolinic acid,apramycin,fusidic acid,chloramphenicol,tetracycline,rifampin,doxycycline and metronidazole.

关 键 词：猪红斑丹毒丝菌 16SrRNA 序列分析 分离 鉴定 云南 

分 类 号：S852.617[农业科学—基础兽医学]