姜黄素缓解草酸钙结晶肾损伤的机制研究  

The Mechanism Research of Curcumin in Alleviating the Renal Injury Induced by Calcium Oxalate Crystals

作　　者：李银辉 谌卫 袁继行 张杰 侯颉玢 郭志勇 LI Yinhui;CHEN Wei;YUAN Jihang(Department of Nephrology,Changhai Hospital,Second Military Medical University,Shanghai(200433))

机构地区：[1]海军军医大学附属长海医院肾内科,上海200433 [2]海军军医大学遗传研究所,上海200433

出　　处：《中国中西医结合肾病杂志》2018年第10期852-855,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国家自然科学基金资助项目(No.81573759;81403261)

摘　　要：目的:观察姜黄素在草酸钙结晶性肾损伤过程中的作用,并初步探讨其可能的机制。方法:在乙醛酸盐腹腔注射诱导C57BL/6J小鼠和一水草酸钙(COM)刺激人肾小管上皮细胞(HK-2)的体内外草酸钙结晶肾损伤模型上,分别加用姜黄素进行干预,应用冯库萨染色观察肾组织草酸钙结晶沉积情况,应用qRT-PCR及Western-blot、免疫组化检测上皮细胞间充质转分化(epithelial-mesenchymal transition,EMT)标志物E-cadherin、N-cadherin和Vimentin在mRNA和蛋白质水平的表达情况。结果:(1)冯库萨染色显示姜黄素显著减少小鼠模型肾草酸钙结晶的沉积。(2)肾组织免疫组化和HK-2细胞qRT-PCR和Western-blot结果显示草酸钙结晶显著降低肾小管上皮细胞的上皮标志物E-cadherin表达和上调间质标志物N-cadherin及Vimentin表达,应用姜黄素干预后明显抑制以上改变。结论:姜黄素可能通过抑制草酸钙结晶所致肾小管上皮细胞EMT改变,从而缓解草酸钙结晶所致肾损伤。Objective:To observe the effect of curcumin in kidney injury induced by calcium oxalate crystals,and to investigate the underlying mechanism of renoprotective effect of curcumin.Methods:C57BL/6J mice intraperitoneally injected with glyoxylate and HK-2 cells exposed to calcium oxalate monohydrate were taken as calcium oxalate-induced kidney damage models.Curcumin was added as the interventional factor respectively.V0N KOSSA staining was used to observe renal oxalate calcium deposition,and immunochemistry、qRT-PCR and Western blot were used to monitor the changes of epithelial-mesenchymal transition(EMT)markers(E-cadherin、N-cadherin and Vimentin).Results:(1)VON KOSSA staining showed that curcumin could significantly reduce the black calcium deposition in mouse kidney tissue.(2)Immunochemistry、qRT-PCR and Western blot witnessed that calcium oxalate decreased the expression of E-cadherin and increased the expression of N-cadherin as well as Vimentin in vivo and in vitro.However,curcumin remarkably alleviated these changes showing above of EMT markers.Conclusion:Curcumin can reduce the glyoxylate induced kidney calcium crystal formation and deposition,and the possible mechanism may be related the regulatory effects on EMT.

关 键 词：草酸钙结晶 姜黄素 EMT 肾结石 

分 类 号：R285.5[医药卫生—中药学]