黑豆汁制何首乌中大豆苷元含量测定  

Study on the content of Daidzein in Polygonum multiflorum Thunb with black bean juice

作　　者：邓青[1] 李霓冰[1] DENG Qing;LI Nibing(Department of Pharmacy,First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,Guangdong,China,510405)

机构地区：[1]广州中医药大学第一附属医院药学部,广东广州510405

出　　处：《分子诊断与治疗杂志》2018年第6期422-427,共6页Journal of Molecular Diagnostics and Therapy

摘　　要：目的通过测定黑豆汁制何首乌中大豆苷元的含量对其质量进行评价。方法采用薄层色谱法对黑豆汁制何首乌、生何首乌、清蒸制何首乌及对照品中的大豆苷元进行定性分析;采用高效液相色谱法对其大豆苷元含量进行定量分析。结果黑豆汁制何首乌薄层色谱中的荧光斑点与大豆苷元对照品色谱的荧光斑点位置对应;而生何首乌、清蒸制何首乌色谱中,与大豆苷元对照品相应的位置上不显相同颜色的荧光斑点。通过高效液相色谱测定得到大豆苷元进样量X(μg)与其对应的峰面积值Y的回归方程为:Y=12.647X+197.35(r=0.999 9),线性范围为1.65~16.56μg,平均回收率98.72%(n=5,RSD=1.54%)。黑豆汁制何首乌大豆苷元含量为(12.34±1.26)mg/g;在生何首乌、清蒸制何首乌中未检测出大豆苷元。结论通过对黑豆汁制何首乌中大豆苷元的鉴定,可有效区分黑豆制何首乌与其它炮制品,从而达到对制何首乌质量评价及鉴别的目的。Objective To investigate daidzein content in Polygonum multiflorum Thunb with black bean juice and to evaluate the quality of Radix Polygoni Multiflori preparata.Methods The daidzein in Polygonum multiflorum Thunb with black bean juice was qualitatively analyzed by thin-layer chromatography(TLC).The daidzein content in Polygonum multiflorum Thunb with black bean juice was carried out by the high performance liquid chromatography(HPLC).Results During TLC,the fluorescent spots of Polygonum multiflorum Thunb with black bean juice could be observed in the corresponding position of daidzein control,while fluorescent spots could not found in the corresponding position on TLC of raw Polygonum multiflorum and steamed Radix Polygoni Multiflori.The regression equation of X daidzein injection volume X(μg)determined by HPLC with its corresponding peak area value Y was obtained,which was Y=12.647X+197.35,r=0.999.The linear range was 1.65μg^16.56μg,and the average recovery rate was 98.72%(n=5,RSD=1.54%).The content of daidzein was 12.34±1.26 mg/g in Polygonum multiflorum Thunb with black bean juice,while daidzein was not detected in raw Polygonum multiflorum Thunb and steamed Radix Polygoni multiflori.Conclusion The identification of daidzein in Polygonum multiflorum Thunb with black bean juice can be used to identify Polygonum multiflorum Thunb with black bean juice from other processed products of Polygonum multiflorum Thunb effectively,so as to evaluate the quality of Radix Polygoni Multiflori preparata.

关 键 词：黑豆汁 大豆苷元 何首乌 

分 类 号：R283.1[医药卫生—中药学]