山羊角中巯基的测定及山羊角对体内巯基的干预研究  

Determination of Thiol in Caprae Hircus Cornu and Its Effect on Thiol Level in Rat Plasma

作　　者：刘睿[1,2,3] 陈建亚 朱振华 张燕[5] 钱大玮 段金廒[1,3] LIU Rui;CHEN Jian ya;ZHU Zhen hua;ZHANG Yan;QIAN Da wei;DUAN Jin ao(Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing,210023,China;Jiangsu Key Laboratory of Research and Development in Marine Bio Resource Pharmaceutics,Nanjing,210023,China;Jiangsu Key Laboratory for High Technology Research of TCM Formulae,Nanjing University of Chinese Medicine,Nanjing,210023,China;Jiangsu Life Science Park Development Co.LTD,Nanjing,210033,China;;School of Public Administration,Nanjing Normal University,Nanjing,210023,China)

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心,中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023 [2]江苏省海洋药用生物资源研究与开发重点实验室,江苏南京210023 [3]江苏省方剂高技术研究重点实验室,江苏南京210023 [4]江苏生命科技创新园,江苏南京210033 [5]南京师范大学公共管理学院,江苏南京210023

出　　处：《南京中医药大学学报》2018年第6期617-620,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏高校优势学科建设工程资助项目(PAPD);江苏省高等学校自然科学研究重大项目(17KJA360005);江苏省高校“青蓝工程”优秀青年骨干教师(2016年);江苏省“333工程”第三层次(2016年);江苏省社会科学基金(15ZXC004)

摘　　要：目的测定山羊角提取液及大鼠血浆中游离巯基(-SH)与总-SH的含量,探讨山羊角对大鼠体内-SH干预情况。方法采用Ellman法测定山羊角提取液中游离-SH与总-SH的含量;大鼠连续灌胃给予山羊角提取物后,测定大鼠血浆中游离-SH和总-SH的水平;大鼠灌胃7d给予山羊角提取物后,连续测定给药后大鼠血浆中游离-SH含量随时间变化情况。结果山羊角提取液中的游离-SH含量介于3.96~5.08mmol/L,总-SH的含量介于4.52~5.94mmol/L;连续给予大鼠山羊角提取液后,第4天始,大鼠血浆中游离-SH与总-SH水平相较于正常大鼠显著提升(P<0.01),而阿司匹林对大鼠血浆中的游离-SH与总-SH水平无影响;口服给予大剂量山羊角后30min,大鼠血浆中游离-SH含量即达最大值(10.16±1.18)mmol/L,8h后大鼠血浆中游离-SH含量基本降至正常大鼠水平。结论山羊角提取液中含有丰富的-SH,且口服山羊角后血浆游离-SH与总-SH水平均显著提升,提示山羊角中含SH基团的一类成分可能为山羊角重要功效物质基础,为角类动物药功效物质基础及效应特点研究提供借鉴与思路。OBJECTIVE To determine the level of native thiols(-SH)and total-SH in goat horn extract and rat plasma.METHODS The thiols levels in goat horn extract and rat plasma were measured using the classical Ellman reagent method.Both the levels of native-SH and total-SH in rat plasma were measured after oral administration of goat horn extract.After oral administration of goat horn extract,the native-SH level in rat plasma was measured,and the curve of-SH level against time after administration was obtained.RESULTS The level of native-SH in goat horn extract ranged from 3.96 to 5.08 mmol/L,and the level of total-SH in goat horn extract ranged from 4.52-5.94 mmol/L.After continuously oral administration of goat horn extract,both the levels of native-SH and total-SH in rat plasma significantly increased(P<0.01)compared with normal rat from d4 to d7.Oral administration of Aspirin did not significantly increase the-SH level in rat plasma.In addition,30 min after oral administration of goat horn extract,the level of native-SH in rat plasma significantly increased to(10.16±1.18)mmol/L,and 8 h later,the level of native-SH in plasma reverted to normal level.CONCLUSION There are abundant-SH contained components in goat horn extract,and goat horn extract could obviously increase the-SH level in rat plasms.The results in the present study suggested that-SH group contained components might be the important material basis of goat horn.It provides a reference for investigations on animal horn derived TCMs.

关 键 词：山羊角 巯基 血浆 测定 

分 类 号：R284.1[医药卫生—中药学]