绿原酸对3T3-L1前脂肪细胞分化的抑制作用  

Inhibition effects of chlorogenic acid on the differentiation of 3T3-L1 preadipocytes

作　　者：杨玉彬[1] 柯斌[1] 秦鉴[2] YANG Yu-bin;KE Bin;QIN Jian(The First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,China;The Seventh Affiliated Hospital of Sun Yat-Men University,Shenzhen 518000,China)

机构地区：[1]中山大学附属第一医院,广东广州510080 [2]中山大学附属第七医院,广东深圳518000

出　　处：《中成药》2018年第11期2380-2385,共6页Chinese Traditional Patent Medicine

基　　金：广东省中医药管理局项目(20181052);广东省自然科学基金项目(2018A030313411)

摘　　要：目的研究绿原酸对3T3-L1前脂肪细胞分化的抑制作用。方法第1天,细胞培养皿中加入不同质量浓度绿原酸(50、100、200μg/m L),以氯化锂为阳性对照。第9天,油红O染色观察细胞内脂质沉积情况,生化法检测细胞内甘油三酯(TG)水平,Western blot、RT-PCR法测定PPARγ2、a P2、LPL mRNA和蛋白表达,免疫荧光染色观察PPARγ2在细胞浆及细胞核中的表达,CCK-8法分析绿原酸对3T3-L1细胞的细胞毒作用。结果绿原酸无细胞毒作用。随着绿原酸质量浓度增加,脂质沉积逐渐减少,TG水平逐渐下降,细胞核中PPARγ2蓝色荧光逐渐变淡。与诱导分化组比较,绿原酸组(50、100、200μg/m L) PPARγ2、a P2、LPL mRNA和蛋白表达显著降低(P <0. 05),并呈量效关系。结论绿原酸可抑制3T3-L1前脂肪细胞分化,可能是该成分抑制脂肪生成、安全有效减肥的机制。AIM To study the inhibition efects of chlorogenic acid on the diferentiation of 3T3-L1 preadipo?cytes.METHODS Different concentrations of chlorogenic acid(50、100、200"ggmL)were added into cell culture plate on the 1st day,lithium chloride was taken as a positive control.On the 9th day,oil red 0 staining was applied to investigating intracellular lipid storage,biochemical metliod was used to detect intracellular triglyceride(TG)level,Western blot and RT-l^CR metliods were adopted in the(PPARy2,aP2 and LPL)mRNA and protein expression determination,immunofluorescence staining was used for obsering PPAR72 expressions in cytoplasm and nucleus,and the cytotoxicity of chlorogenic acid on 3T3-L1 cells was analyzed by CCK-8 metiiod.RESULTS Chlorogenic acid exhibited no cytotoxicity.With the increase of chlorogenic acid concentration,gradualy re?duced lij^id storage,gradually decreased TG level,and gradually diluted PPAR#blue fluorescence in nucleus were found.Compared with the induced differentiation group,chlorogenic acid groups(50、100、200"ggmL)demonstrated significantly reduced PARy2,aP2 and LPL mRNA and protein expressions(P<0.05)in a dose-effect relationship.CONCLUSION Chlorogenic acid can inhibit the differentiation of 3T3-L1 preadipocytes,which may be this constituent’s mechanisms of inhibiting lipogenesis,and effectively,safely losing weight.

关 键 词：绿原酸 3T3-L1前体脂肪细胞 分化 

分 类 号：R966[医药卫生—药理学]