大败毒胶囊质量标准的研究  

Quality standard for Dabaidu Capsules

作　　者：高燚 李慧[1] 陈闻燕 黄胜良 钱晓华 张丽[1] GAO Yi;LI Hui;CHEN Wen-yan;HUANG Sheng-liang;QIAN Xiao-hua;ZHANG Li(Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Renshou Pharmaceutical Co.,Ltd.,Huaian 223200,China)

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏仁寿药业有限公司,江苏淮安223200

出　　处：《中成药》2018年第11期2450-2455,共6页Chinese Traditional Patent Medicine

基　　金：江苏省高等学校大学生创新创业训练计划(201510315051Z);江苏仁寿药业有限公司委托合作(2014062)

摘　　要：目的建立大败毒胶囊(大黄、蒲公英、陈皮等)的质量标准。方法 TLC法定性鉴别大黄、黄柏、赤芍、白芷、陈皮、乳香。HPLC法测定芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚含有量。结果 TLC斑点清晰,分离度好,阴性无干扰。芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0. 19~5. 93、0. 64~20. 36、0. 16~5. 25、0. 15~4. 91、0. 19~6. 06μg/m L范围内线性关系良好(r≥0. 999 6),平均加样回收率分别为97. 12%(RSD=2. 33%)、100. 41%(RSD=2. 81%)、102. 42%(RSD=2. 02%)、99. 24%(RSD=1. 60%)、97. 08%(RSD=2. 98%)。结论该方法简便、可靠、准确,可用于大败毒胶囊的质量控制。AIM To establisli the quality standard for Dabaidu Capsules(Rhei Radi%et Rhizoma,Taraxaci Herba,Citri reticulatae Pericarpium,etc.).METHODS TLC was used for the qualitative identification of Rhei Radix et Rhizoma,Phellodendri chinensis Cortex,Paeoniae Radii Rubra,Angelicae dahuricae Radii,Citri reticulatae Pericarpium ad Olibanum.HPLC was adopted in the contentdetermination of aloe-emodin,rhein,emodin,chrsophanol and physcion.RESULTS The clear and well-separated TLC spots were free from negative interference.Aloe-emodin,rhein,emodin,chrysophanol,physcion showed good linear relationships wit%iin the ranges of 0.19-5.93,0.64-20.36,0.16-5.25,0.15-4.91,0.19-6.06ug/mL(r)0.999 6),whose average recovvries were 97.12%(RSD=2.33%),100.41%(RSD=2.81%),102.42%(RSD=2.02%),99.24%(RSD=1.60%),97.08%(RSD=2.98%),respectively.CONCLUSION This simp%,reliable and accurate metiiod can be used for the quality control of Dabaidu Capsules.

关 键 词：大败毒胶囊 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 TLC HPLC 

分 类 号：R927.2[医药卫生—药学]