HPLC法同时测定益母草中4种成分  

Simultaneous determination of four constituents in Leonurus japonicas

作　　者：乔晶晶 吴啟南 许一鸣 薛敏 王倩 赵玲玲 QIAO Jing-jing;WU Qi-nan;XUYi-ming;XUE Min;WANG Qian;ZHAO Ling-ling(Jiangsu Province Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engi/ering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Colle^,e of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《中成药》2018年第11期2467-2471,共5页Chinese Traditional Patent Medicine

基　　金：中医药行业科研专项(2015468002-3);江苏高校优势学科建设工程资助项目(yxk-010);江苏省中药资源产业化过程协同创新中心(2011)。

摘　　要：目的建立HPLC法同时测定益母草Leonurus japonicas Houtt.药材中盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷的含有量。方法益母草70%乙醇提取液的分析采用HPLC-PDA、Waters XBridgeTMC18色谱柱(4. 6 mm×250 mm,5μm);以甲醇-水(含0. 1%甲酸)为流动相;体积流量1. 0 m L/min;检测波长277 nm;柱温30℃。采用灰色关联度分析(GRA)对其进行综合评价。结果盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷分别在3. 73~466 (r=1. 000 0)、0. 40~198. 4 (r=0. 999 9)、0. 50~47. 92 (r=0. 999 4)、1. 83~109. 68 (r=0. 999 6)μg/m L的范围内线性关系良好,平均加样回收率分别为98. 25%、99. 46%、98. 61%、98. 91%,RSDs分别为1. 97%、2. 67%、2. 19%、2. 17%。同一产地中的不同成分、不同产地中的同一成分均存在一定差异,河南-南阳的益母草样品综合品质评价最优。结论该方法准确稳定,重复性好,可用于益母草的质量控制。AIM To establisli an HPLC metliod for simultaneous determination of leonurine hydrochloride,ru?tin,hyperoside and isoquercetin in Leonurus japonicas Houtt..METHODS The analysis of 70%ethanol extract was performed on a 30℃Waters‘Bridge?C18 column(4.6 mm X250 mm,5um),witii the mobile phase com?prising of metiianol-water(containing 0.1%formic acid)flowing at 1.0 mLmin in a gradient elution manner,and the detection wavelength was set at 277 nm.Grey relational analysis(GRA)was used to comprehensively e-valuate the quality of the samples.RESULTS Leonurine hydrochloride,rutin,hyperoside and isoquercetin showed good linear relationships within the ranges of 3.73-466(r=1.000 0),0.40-198.4(r=0.999 9),0.50-47.92(r=0.999 4),1.83-109.68(r=0.999 6)ug/mL,whose average recoveries were 98.25%,99.46%,98.61%,98.91%witii the RSDs of 1.97%,2.67%,2.19%,2.17%,respectively.There were some diferences in components in L.japonicas from the same region and the same component in L.japonicas from diferemt regions.The results of comprehensive evaluation showed that L.japonicas from Nanyang City of Henan Province were the samples witii the best quality.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of L.japonias.

关 键 词：益母草 盐酸益母草碱 芦丁 金丝桃苷 异槲皮苷 HPLC 

分 类 号：R284.1[医药卫生—中药学]