干旱胁迫对草石蚕保护酶活性和渗透调节物质的影响  

Effects of Drought Stress on Protective Enzyme Activity and Osmotic Adjustment Substances of Stachys Sieboldii Miq.

作　　者：班甜甜 张素勤[2] 肖体菊 徐彦军[2] 耿广东[2] BAN Tian-tian;ZHANG Su-qin;XIAO Ti-ju;XU Yan-jun;GENG Guang-dong(Horticultural Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;College of Agriculture,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州省农业科学院园艺研究所,贵州贵阳550006 [2]贵州大学农学院,贵州贵阳550025

出　　处：《江西农业大学学报》2018年第5期956-964,共9页Acta Agriculturae Universitatis Jiangxiensis

基　　金：贵州省科技厅重大专项计划(黔科合重大专项字[2016]3002号);贵州省农业攻关项目(黔科合支撑[2016]2603号)~~

摘　　要：以抗旱性不同的3个草石蚕品种为试材,研究干旱胁迫下草石蚕保护酶活性和渗透调节物质的变化规律,分析不同抗旱性品种在干旱胁迫下的生理变化,以期为草石蚕的抗旱性鉴定提供参考依据,并进一步为筛选和选育抗旱性品种提供理论基础。在设施内采用自然干旱胁迫方法,分别胁迫4,8,12和16 d,通过测定各生理指标的数据,研究干旱胁迫对草石蚕的影响。结果表明:随干旱胁迫时间延长,3个品种的可溶性糖和Pro含量逐渐增加;"贵栽1号"可溶性蛋白含量一直升高,而"鲁引1号"和"赣引1号"则呈先升高后降低的变化趋势; SOD、POD和CAT活性逐渐升高;膜脂过氧化产物MDA含量逐渐增加。抗旱性强的"贵栽1号"可溶性糖、Pro和MDA含量增加幅度最低,SOD、POD和CAT活性升高幅度最大。草石蚕保护酶系统、渗透调节物质和膜脂过氧化产物的变化规律与干旱胁迫程度和品种抗旱性有关。Three varieties of s tachys Sieboldii Miq.with different drought resistance were used to investigate the effect of drought stress on protective enzyme activity and osmotic adjustment substances and analyze the changes in physiological properties under drought stress.The experiment could provide theoretical basis for selecting the varieties of s tachys Sieboldii Miq with drought resistance.The effects of drought stress on s tachys Sieboldii Miq which was treated by natural drought stress for 4,8,12,and 16 days were studied by using the data of physiological indexes in the present study.The results showed that the content of soluble sugar,Pro and MDA rose gradually,and the activities of SOD,POD and CAT kept rising all the time under drought stress.The content of soluble sugar,Pro and MDA of the variety“Guizai NO.1”which had good drought resistance had the smallest increasing amplitude.While the activities of SOD,POD and CAT of“Guizai NO.1”had the largest increasing amplitude.The content of soluble protein of the varieties“Luyin No.1”and“Ganyin No.1”rose in the first stage,and then fell under drought stress.While the content of soluble protein of“Guizai NO.1”kept rising all the time.It could be concluded that the protective enzyme system and osmotic adjustment substance were affected by drought in stachys sieboldii Miq.stress,and the degree of the effect was related to the drought resistance of varieties.

关 键 词：草石蚕 干旱胁迫 抗旱性 保护酶系统 渗透调节物质 

分 类 号：Q945.78[生物学—植物学]