Chinese 1型弓形虫在终宿主家猫肠内发育的研究  

Research of the development of Chinese 1 strain Toxoplasma gondii in intestinal epithelial cells of cat

作　　者：祝子伏 董辉[1] 苏瑞景 陆瑶瑶[1] 王梦瑶 杨玉荣[1] ZHU Zi-fu;DONG Hui;SU Rui-jing;LU Yao-yao;WANG Meng-yao;YANG Yu-rong(College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450000,China)

机构地区：[1]河南农业大学牧医工程学院兽医病理教研室,郑州450000

出　　处：《中国人兽共患病学报》2018年第10期883-887,共5页Chinese Journal of Zoonoses

基　　金：中国博士后科学基金(No.2016M600577);河南省高校科技创新人才支持计划(No.17HASTIT038);河南省科技攻关(No.182102110038)资助的课题联合资助~~

摘　　要：目的观察研究TgCatCHn4(Chinese 1)株弓形虫在猫回肠上皮细胞发育过程中的虫体形态特点,对小鼠RH株弓形虫急性感染期的病理变化和虫体分布进行探讨。方法复制弓形虫感染的终末宿主和中间宿主模型,采用石蜡切片H&E染色和IHC染色方法。结果家猫摄入弓形虫包囊后,可成功排泄弓形虫卵囊(3DPI^10DPI)。回肠病理变化主要表现为吸收细胞脱落和固有层淋巴细胞的坏死,可观察到弓形虫在回肠上皮裂殖生殖和配子生殖各阶段虫体的形态。RH株弓形虫感染的小鼠病理损伤主要表现为肝脏、脾脏和淋巴结的局灶性坏死,坏死灶分布多量速殖子,虫体多以花瓣状、簇状分布。结论详细探讨了弓形虫感染猫和小鼠后的虫体形态学和对机体的病理损伤特点,为弓形虫在终末宿主和中间宿主的病理诊断和入侵机制研究提供参考依据。Definitive host and intermediate host of Toxoplasma gondii infection were replicated in this study.The morphology of TgCatCHn4(Chinese 1)strains T.gondii development in cat ilem epithelial cells was observed by paraffin section,Hematoxylin-Eosin staining and immunohistochemistry methods.The pathological lesions and the distribution of T.gondii tachyzoites in mice were observed during acute toxoplasmosis induced by RH strains T.gondii.Results showed that after domestic cat ingested cysts of T.gondii,oocysts were shed successfully at 3-10 d post inoculation.Desquamation of absorptive cells and lymphocytes necrosis in lamina propria were observed in jejunum of cat.Multiplicative reproduction of schizonts and gametes of T.gondii were found in the ilem epithelial cells.Multiple focal necroses were observed in liver,spleen and mesenteric lymph nodes of mice induced by RH strains T.gondii.A large number of T.gondii tachyzoites were grew in necrotic lesions.The tachyzoites were crowed together and arranged petal shaped.The lesions induced by T.gondii in cat model and mice model were discussed in this paper,also focus the morphology of T.gondii tachyzoites,schizonts and gametes,this results provide the reference base for pathological diagnosis and invasion mechanism research in definitive host and intermediate hosts.

关 键 词：弓形虫 RH株 TgCatCHn4株 猫 小鼠 虫体形态 病理损伤 

分 类 号：R382.5[医药卫生—医学寄生虫学]