响应曲面法优化钩藤叶中钩藤碱和异钩藤碱的提取条件  

Optimization on extracting conditions of rhynchophylline and isorhynchophylline in Uncaria rhynchophylla leaves by response surface method

作　　者：徐佳瑜 王晓红[1] 杨胜伟 吕享[1] 张智仙 张明生[1] XU Jiayu;WANG Xiaohong;YANG Shengwei;LYU Xiang;ZHANG Zhixian;ZHANG Mingsheng(College of Life Sciences,State Engineering Technology Institute for Karst Desertfication Control,Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guizhou University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州大学生命科学学院/国家喀斯特石漠化防治工程技术研究中心/山地植物资源保护与种质创新教育部重点实验室,贵州贵阳550025

出　　处：《中南林业科技大学学报》2018年第10期131-138,共8页Journal of Central South University of Forestry & Technology

基　　金：国家重点研发计划课题(2016YFC0502604);贵州省科技计划重大专项课题(黔科合平台人才[2017]5411-06);国家喀斯特石漠化防治工程技术研究中心建设(2012FU125X13);贵州省科技创新人才团队建设专项资金(黔科合平台人才[2016]5624);贵州省教育厅创新群体重大研究项目(黔教合KY字[2016]023)

摘　　要：以钩藤叶为材料,在干燥温度、提取时间和提取剂浓度3个单因素试验的基础上,采用Box-Behnken中心组合进行3因素3水平试验设计,建立回归方程,优化出钩藤叶中钩藤碱和异钩藤碱的最佳提取条件。模拟得到钩藤叶干燥温度为54.95℃时,钩藤碱和异钩藤碱的最优提取条件为超声时间60 min、甲醇浓度73.35%。在此条件下,钩藤碱的最高提取率为133.342μg·g^(-1),异钩藤碱的最高提取率为429.746μg·g^(-1)。为了方便实际操作,校正条件为干燥温度55℃、超声提取60 min、甲醇浓度73%。通过验证试验得到钩藤碱平均提取率为130.8μg·g^(-1),异钩藤碱平均提取率为425.6μg·g^(-1),与模型预测值较为接近。因此响应曲面法可以对钩藤叶中钩藤碱和异钩藤碱的提取条件进行优化。The leaves of U.rhynchophylla were used as test materials,on the basis of three single factor tests,the effects of drying temperature,extracting time and extractant concentration were studied by three-factor and three-level test developed by Box-Behnken central composite test design and the relative equations were established,which optimized the extraction conditions.When the drying temperature of leaves is 54.95℃,the optimal extraction conditions of rhynchophylline and isorhynchophylline were 60 mins of ultrasound time,73.35%of methanol,and the extraction value of rhynchophylline was 133.342μg·g-1 and of isorhynchophylline was 429.746μg·g-1.In order to facilitate the practice,the conditions were modified as follows:drying temperature was 55℃,ultrasound time was 60 min,the volume of methanol was 73%.From the verification test,the average yield of rhynchophylline was 130.8μg·g-1 and of isorhynchophylline was 425.6μg·g-1,which is close to the model predicted value.Thus the response surface method can be used to optimize the extraction conditions of rhynchophylline and isorhynchphylline in the leaves of U.rhynchophylla.

关 键 词：钩藤 钩藤碱 异钩藤碱 提取条件 响应曲面法 

分 类 号：S789.9[农业科学—林学]