黄芪丹参配伍提取物对大鼠心肌梗死后心室重构的影响  

Effects of Astragalus and Salvia Miltiorrhiza Extract on Ventricular Remodeling after Myocardial Infarction in Rats

作　　者：张瓅方[1] 高丽[1] 李超 杨雷[1] 张仲鸣 毛秉豫[1] ZHANG Li-fang;GAO Li;LI Chao;YANG Lei;ZHANG Zhong-ming;MAO Bing-yu(Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation,Nanyang Institute of Technology,Nanyang 473004,China)

机构地区：[1]南阳理工学院河南省张仲景方药与免疫调节重点实验室,南阳473004

出　　处：《科学技术与工程》2018年第30期145-149,共5页Science Technology and Engineering

基　　金：国家自然科学基金(81173372)资助

摘　　要：观察黄芪、丹参配伍提取物对大鼠心肌梗死后心室重构的影响,分析其保护机制。雄性SD大鼠采用冠状动脉左前降支结扎法复制心肌梗死大鼠模型,随机分为假手术组、模型组、黄芪、丹参配伍提取物低、中、高剂量组(10、20、40 mg·kg^(-1)·d^(-1))、卡托普利组、每组8只。各组每日采用对应药物剂量灌胃给药,28 d后采用心脏超声检查心功能情况,ELISA法测定心肌组织中脑钠尿肽(BNP)及转化生长因子-β(TGF-β)。黄芪、丹参配伍提取物高剂量组与模型组相比,可改善心脏功能,其中BNP和TGF-β含量明显降低(P <0. 05)。说明黄芪、丹参配伍提取物可以提高心梗后心室舒缩功能,增加供血量,降低心肌纤维化、有效抑制心室重构。To observe the effects of Astragalus and Salvia miltiorrhiza extract on ventricular remodeling after myocardial infarction in rats and analyze the protective mechanism,the rat model of myocardial infarction was replicated by ligation of left anterior descending branch of coronary artery in male SD rats.The rats were randomly divided into sham operation group,model group,Astragalus membranaceus and Salvia miltiorrhiza in low,medium and high dose group(10,20,40 mg·kg-1·d-1),Kato Pury group and 8 rats in each group.Each group was administered every day with the corresponding dose of the drug.After 28 d,the cardiac function was examined by echocardiography.The ELISA method was used to determine the brain natriuretic peptide(BNP)and transforming growth factor beta(TGF-beta)in the myocardium.Compared with the model group,the high dose group of Astragalus and Salvia miltiorrhiza could improve the cardiac function,and the contents of BNP and TGF-beta decreased significantly(P<0.05).It is concluded that astragalus membranaceus and Salvia miltiorrhiza extract can improve ventricular systolic and diastolic function after myocardial infarction,increase blood supply,reduce myocardial fibrosis,and effectively inhibit ventricular remodeling.

关 键 词：黄芪、丹参配伍提取物 心肌梗死 心室重构 BNP TGF-Β 

分 类 号：R285.5[医药卫生—中药学]