苏丹红Ⅰ对大鼠十二指肠CYP 1A1和CYP 2E1基因表达的影响  

Effects of Sudan Ⅰ on Expressions of CYP 1A1 and CYP 2E1 Genes in Duodenum of Rats

作　　者：单春兰 刘超英[1] 孙文汇[1] 富国文[1] 高洪 严玉霖 景麟稀 SHAN Chun-lan;LIU Chao-ying;SUN Wen-hui;FU Guo-wen;GAO Hong;YAN Yu-lin;JING Lin-xi(College of Animal Science and Technology,Yunnan Agricultural University,Kunming,Yunnan,650201,China;College of Animal Medical,Yunnan Agricultural University,Kunming,Yunnan,650201,China)

机构地区：[1]云南农业大学动物科技学院,云南昆明650201 [2]云南农业大学动物医学院,云南昆明650201

出　　处：《动物医学进展》2018年第11期58-62,共5页Progress In Veterinary Medicine

基　　金：云南省现代农业生猪产业技术体系建设项目(云财农[2010]119号);云南省高校科技创新团队支持计划资助项目(云教科[2011]14号);云南省高端科技人才引进计划项目(2009CI125)

摘　　要：探讨苏丹红Ⅰ(SudanⅠ)对大鼠十二指肠细胞色素P4501A1(CYP 1A1)和细胞色素P450 2E1(CYP2E1)表达的影响。将30只清洁级SD大鼠随机分为2组,对照组(C组)和SudanⅠ处理组(包括4个剂量组:Ⅰ组265mg/kg,Ⅱ组530mg/kg,Ⅲ组795mg/kg,Ⅳ组975mg/kg),连续饲喂10d,采集十二指肠作为检测样品,应用免疫组化染色技术(IHC)与原位杂交技术(ISH)研究SudanⅠ对十二指肠CYP 1A1、CYP 2E1表达的影响。结果显示,SudanⅠ处理组十二指肠CYP 1A1、CYP 2E1基因表达的积分光密度极显著高于C组(P<0.01),且在一定剂量范围内数据呈现上升趋势。说明SudanⅠ能够诱导CYP 1A1、CYP 2E1的表达增加,从而发挥各种毒性效应,最终导致肠道损伤。In order to explore the effect of SudanⅠon CYP 1A1 and CYP 2E1 expressions in rat duodenum,experimental animals were randomly divided into two groups:control group,SudanⅠtreatment groups(four doses of treatment:Ⅰgroup 265 mg/kg,Ⅱgroup 530 mg/kg,Ⅲgroup 795 mg/kg andⅣgroup 975 mg/kg).All rats were killed and their duodenums were collected for detecting after ten days.The immunohistochemistry(IHC)and in situ hybridization(ISH)methods were use to analyze the changes of CYP 1A1 and CYP 2E1 expressions in rat duodenum.The result showed that IOD of CYP 1A1 and CYP 2E1expressions increased markedly(P<0.01)in duodenums in SudanⅠtreatment groups and showed uptrend in certain dose range.The results indicated that SudanⅠcan up-regulate the expressions of CYP 1A1 and CYP 2E1,and educe various kinds of poisoning effects.Finally,SudanⅠcan lead to intestine injury.

关 键 词：苏丹红Ⅰ 十二指肠 细胞色素1A1 细胞色素2E1 

分 类 号：S859.84[农业科学—临床兽医学]