鸡传染性支气管炎病毒江苏分离株S1基因的克隆与序列分析  

Cloning and Sequence Analysis of S1 Gene of Avian Infectious Bronchitis Virus Isolated from Jiangsu Province

作　　者：卢秀娴 张思远 梁昭平 叶贺佳 梅廷媛 LU Xiu-xian;ZHANG Si-yuan;LIANG Zhao-ping;YE He-jia;MEI Ting-yuan(Guangzhou City,South China Agricultural Biological Pharmaceutical Co.,Ltd.,Guangzhou,Guangdong,510300,China)

机构地区：[1]广州市华南农大生物药品有限公司,广东广州510300

出　　处：《动物医学进展》2018年第11期84-87,共4页Progress In Veterinary Medicine

摘　　要：从江苏省某肉鸡养殖场发生疑似传染性支气管炎的发病鸡群中分离到1株鸡传染性支气管炎病毒,命名为CK/JS/073/2017。参照GenBank中IBV的核苷酸序列,设计特异性和全长序列引物,应用RT-PCR技术对CK/JS/073/2017分离株的S1基因进行克隆与序列测定,并进行比对分析。结果显示,CK/JS/073/2017的S1基因片段大小为1 635bp,编码545个氨基酸,蛋白裂解识别位点序列为HRRRR,与中国大部分分离毒株相一致。CK/JS/73/2017株与国内外参考毒株核苷酸同源性为64.7%~99.5%,与位于同一基因型的参考毒株间同源性较高,与我国使用的常规Mass型疫苗毒株及其他参考株同源性较低。以疫苗株H120为参照,该分离株S1基因变异程度较大,出现了基因突变、缺失或插入等现象。An avian infectious bronchitis virus strain was isolated from a flock of suspected infectious bronchitis in a broiler farm in Jiangsu Province in May 2017,named CK/GD/073/2017.The specific and full-length sequence primers were designed according to the nucleotide sequence of IBV in GenBank.The S1 gene of CK/JS/073/2017 isolate was cloned,sequenced and compared by PT-PCR.The results showed that the size of the S1 gene fragment of CK/JS/073/2017 is 1 635 bp,encoding 545 amino acids,and the sequence of the proteolytic recognition site is HRRRR,which is consistent with most isolated strains in China.The nucleotide homology between the CK/JS/73/2017 strain and the reference strains at home and abroad is 64.7%-99.5%,and the homology between the strains of the same genotype is higher and the genetic relationship is closer.The reference strains belong to different branches and are closely related,but have low homology with the conventional Mass vaccine strains used in China.Taking the vaccine strain H120 as a reference,the S1 gene has a large degree of variation,and there are phenomena such as gene mutation,deletion or insertion.

关 键 词：鸡传染性支气管炎病毒 分离 鉴定 S1基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]