咪喹莫特对哮喘大鼠血清TIM-3及肺组织CXCR4表达的影响  

Effects of imiquimod on the expression of serum Tim-3 and CXCR4 in lung tissue of rats with asthma

作　　者：杨丽霞[1] 贾钦尧 蒋莉[1] 董琼[1] YANG Lixia;JIA Qinyao;JIANG Li;DONG Qiong(Department of Respiratory,Nanchong Central Hospital,Nanchong 637000,Sichuan,China;School of Pharmacy,North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院第二临床医学院.南充市中心医院呼吸内科,四川南充637000 [2]川北医学院药学院药物研究所,四川南充637000

出　　处：《西部医学》2018年第11期1570-1572,1578,共4页Medical Journal of West China

基　　金：四川省卫计委科研课题(150092)

摘　　要：目的探讨咪喹莫特对哮喘大鼠血清TIM-3及肺组织CXCR4表达的影响。方法选取雌性BALB/C纯系清洁级大鼠18只,随机分为空白组、模型组及观察组各6只;空白组采用生理盐水处理,模型组使用OVA制造哮喘大鼠模型,观察组在空白组基础上采用咪喹莫特治疗;结束后检测大鼠血清TIM-3水平;采用RT-PCR检测肺组织基质细胞衍生因子1(SDF-1)、趋化因子受体4(CXCR4)及β-actin mRNA水平;测定支气管管腔内周长、支气管平滑肌面积、管壁面积及平滑肌细胞核数。结果 3组大鼠支气管壁面积、支气管平滑肌厚度及平滑肌细胞核数有显著差异(P<0.05),且观察组大鼠支气管壁面积、支气管平滑肌厚度及平滑肌细胞核数均显著低于模型组(P<0.05);3组大鼠血清TIM-3水平亦有显著差异(P<0.05),且观察组显著低于模型组(P<0.05);3组大鼠肺组织SDF-1及CXCR4表达量亦有显著差异(P<0.05),且观察组肺组织SDF-1及CXCR4表达量显著低于模型组(P<0.05)。结论咪喹莫特对哮喘大鼠干预后可显著降低大鼠血清TIM-3水平,并改善肺组织CXCR4表达,具有较高应用价值。Objective The effects of imiquimod on the expression of serum Tim-3 and CXCR4 in the lung tissue of the asthmatic rats were analyzed.Methods Female BALB/C rats were randomly divided into control group,model group and observation group.The control group was treated with normal saline.The model group was treated with the OVA model of asthmatic rats.The observation group was treated with imiquimod.The serum TIM-3 was detected.The SDF-1,CXCR4 andβ-actin mRNA were measured.The bronchial lumen perimeter,bronchial smooth muscle area,wall area,numbers of smooth muscle cells.Results There were significantly different among bronchial wall area,bronchial smooth muscle thickness and smooth muscle nuclei,expression of SDF-1,expression of CXCR4 and TIM-3 of blank group,model group and observation group(P<0.05).The bronchial wall area,bronchial smooth muscle thickness and smooth muscle nuclei,expression of SDF-1,expression of CXCR4 and TIM-3 of observation group were lower than that of model group(P<0.05).Conclusion Imiquimod can significantly reduce the level of TIM-3 in serum and improve the expression of CXCR4 in lung tissue,which has a high value of application.

关 键 词：咪喹莫特 哮喘大鼠 TIM-3 CXCR4 

分 类 号：R322.3[医药卫生—人体解剖和组织胚胎学]