辛伐他汀对大鼠缺血随意皮瓣作用时NOS和ICAM-1表达的影响  

Effects of simvastatin on the expression of NOS and ICAM-1 in ischemic random skin flaps in rats

作　　者：刘鸿雁[1] 彭海涛[1] 蒋婷[1] 黄文炼 何清莲[1] LIU Hongyan;PENG Haitao;JIANG Ting;HUANG Wenlian;HE Qinglian(Department of Burn and Plastic Surgery,The Second Affiliated Hospital of North Sichuan Medical College, Central Hospital of Nanchong,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院第二临床医学院.南充市中心医院烧伤整形美容外科,四川南充637000

出　　处：《西部医学》2018年第11期1579-1582,共4页Medical Journal of West China

基　　金：四川省教育厅重点科研项目(17ZA0181)

摘　　要：目的评价辛伐他汀对大鼠随意皮瓣作用时一氧化氮合酶(NOS)及细胞间粘附因子-1(ICAM-1)表达的影响。方法健康清洁级雄性SD大鼠60只,6周龄,体重(220±20)g,随机分为实验组及对照组各30只,背部制作随意皮瓣模型后,分别通过腹腔注射给予辛伐他汀(5mg/kg,实验组)和等容积的生理盐水(对照组),1次/d,7d后大体观察两组皮瓣的成活情况并计算成活率、皮瓣病理变化,切取皮瓣近、中、远3段长0.5cm、宽0.5cm的全厚皮瓣组织匀浆离心后,检测组织中NOS、ICAM-1的含量。结果术后7d,皮瓣中、远段组织学病理切片提示,实验组组织中炎症细胞浸润、坏死程度较对照组轻。实验组皮瓣成活率及NOS活性均较对照组高(P<0.05),ICAM-1活性明显低于对照组(P<0.05)。结论辛伐他汀提高随意皮瓣成活率,其机制可能与辛伐他汀增加NOS生成,抑制ICAM-1表达,进而减轻炎性反应有关。Objective To investigate the effects of simvastatin on the expression of NOS and ICAM-1 in ischemic random skin flaps in rats.Methods 60 SD rats of both males aged 6 weeks weighing 220±20g were randomly divided into two groups with 30 rats in each group,and a random dorsal skin flap was established in each anima1.In the experimental group each rat was given a intraperitoneal injection with simvastatin(5mg/kg)and the control group animals each received the same volume of saline also by intraperitoneal injection,once a day for 7 days.The viable situation and survival rate of all flaps in the two groups were observed,meanwhile pathological changes were observed with light microscope.The full-thickness flaps of tissue in the near,middle and distal segments of the flap with 0.5cm×0.5cm were immediately removed for the determination the expression of NOS and ICAM-1.Results After 7 days after operation,the changes of middle and distal segments of the flap in biopsy tip,the tissue inflammatory cell infiltration and the situation of necrosis in the experimental group were lighter than that in the control group.The survival rates of flaps and the activity of NOS were greatly higher in the experimental group than that in the control group(P<0.05),and the activity of ICAM-1 in the experimental flap was significantly lower than the control group(P<0.05).Conclusion Simvastatin can increase the survival rate of random skin flaps in rats,which may be related to simvastatin increased NOS,inhibition the expression of ICAM-1,thereby reducing inflammatory responses.

关 键 词：辛伐他汀 随意皮瓣 一氧化氮合酶(NOS) 细胞间粘附因子-1(ICAM-1) 大鼠 

分 类 号：R751[医药卫生—皮肤病学与性病学]