屎肠球菌廉价培养基的研制  

The Development of the Low-cost Medium Using for Enterococcus faecium

作　　者：李英英[1] 陈曦[1] 宋铁英[1] LI Ying-ying;CHEN Xi;SONG Tie-ying(Institute of Biotechnology,Fujian Academy of Agricultural Science,Fuzhou,Fujian 350003)

机构地区：[1]福建省农业科学院生物技术研究所,福州350003

出　　处：《安徽农业科学》2018年第32期80-81,90,共3页Journal of Anhui Agricultural Sciences

基　　金：福建省科技计划项目(2017R1019-2;2017R1019-7;2018R1019-4);福建省农科院科技创新项目(A2017-23;PC2017-6);福建省特种水产配合饲料重点实验室开发课题(TMKJZ1705);鳗鲡现代产业技术教育部工程研究中心开放基金(RE201802)

摘　　要：[目的]为了降低屎肠球菌微胶囊制剂的发酵成本,[方法]用自主分离的一株屎肠球菌作为发酵菌株,用玉米和豆粕替代MRS基础培养基中的碳源和氮源,设计不同替代比例的MRS廉价培养基,经过培养和计数,筛选出价格低廉又适合该菌株生长的廉价培养基。[结果]玉米和豆粕作为碳源和氮源制成的替代培养基适合该屎肠球菌生长;结合价格和培养效果,筛选出碳源50%替代、氮源100%替代的MRS-C50N100替代培养基为适合用于屎肠球菌工业化发酵的廉价培养基。[结论]MRS-C50N100替代培养基既可以极大降低屎肠球菌的发酵成本,又可以使活菌数达到益生菌制剂要求的有效活菌量,可用于屎肠球菌微胶囊制剂的工业化生产。[Objective]In order to reduce the fermentation cost of microencapsulated Enterococcus faecium.[Method]The carbon and nitrogen source in MRS basic medium were instead of corn and soybean meal which were usually used as stuff.MRS low-cost medium with different replacement ratio was designed and a strain of E.faecium isolated by ourselves was used as fermentation strain.After incubation and counting,low-cost medium suitable for the growth of the strain was screened out.[Result]The result showed that the low-cost medium,in which corn and soybean meal were used as carbon and nitrogen source,were suitable for the growth of E.faecium.Considering the price and the effect of culture,the substituting medium MRS-C 50 N 100 with 50%alternative carbon source and 100%alternative nitrogen source was selected as a low-cost medium and suitable for industrial fermentation of E.faecium.[Conclusion]MRS-C 50 N 100 can greatly reduce the fermentation cost of E.faecium,and enable to reach the viable count required by probiotics.It can be used in industrial production of microencapsulated E.faecium.

关 键 词：屎肠球菌 MRS 廉价培养基 饲料原料 

分 类 号：S816.6[农业科学—饲料科学]