MEF2A基因第11外显子多态性与颈动脉斑块易损性的关联分析  

Relationship between polymorphisms of MEF2A gene exon 11 and prevalence of vulnerable atherosclerotic carotid plaque

作　　者：王凤[1] 金笑平[1] 王恩[1] 王皖芬[1] 郑周[1] 胡晓飞[1] 何欣威 胡彩虹[2] 应忠明[3] WANG Feng;JIN Xiaoping;WANG En(Department of Neurology,Taizhou Hospital of Zhejiang Province,Taizhou 317000,China)

机构地区：[1]浙江省台州医院神经内科,317000 [2]浙江省台州医院中医科,317000 [3]台州市中西医结合医院神经内科

出　　处：《浙江医学》2018年第21期2316-2320,共5页Zhejiang Medical Journal

基　　金：浙江省自然科学基金项目(LY13H290003);浙江省医药卫生一般研究计划(2016KYA186);台州市科技计划项目(1201KY01)

摘　　要：目的探讨肌细胞增强因子2A(MEF2A)第11外显子多态性与颈动脉斑块易损性的关系。方法共纳入660例脑梗死患者,根据颈动脉超声检查结果分为易损斑块组205例和稳定斑块组455例。采用PCR及PCR产物直接测序技术法检测两组患者MEF2A基因第11外显子区域的基因多态性。结果易损斑块组rs367780642 (A/G)位点GG、GA基因型频率分别为94.15%和5.85%,稳定斑块组分别为96.04%和3.96%,差异无统计学意义(P>0.05);易损斑块组rs759657998位点的CCG/CCG、-/CCG基因型频率分别为91.71%和8.29%,稳定斑块组分别为92.31%和7.69%,差异无统计学意义(P>0.05)。两组患者rs376557691的(CAG)n存在基因多态性,组间比较差异无统计学意义(P>0.05);校正临床资料的差异后,两组间差异仍无统计学意义(P>0.05)。结论 MEF2A基因rs36778064、rs759657998及rs376557691位点基因多态性与浙江地区汉族人颈动脉易损斑块的发生倾向无关。Objective To investigate the relationship between the polymorphisms of MEF2A gene exon 11 and the prevalence of vulnerable atherosclerotic carotid plaque.Methods Two hundred and five patients with vulnerable atherosclerotic carotid plaque and 455 patients with stable plaque were included in the study.The polymorphisms of MEF2A gene exon 11 were genotyped by polymerase chain reaction(PCR)and DNA sequencing.Results In all subjects,both rs367780642(A/G)and rs759657998(-/CCG)had polymorphism,but no statistical difference was found in the genotype distributions of rs367780642 and rs759657998 between vulnerable plaque group and stable plaque group(P>0.05).Distribution frequency of(CAG)n alleles in rs376557691 also had no difference between two groups(P>0.05).Conclusion Our results indicate that the polymorphisms of MEF2A gene rs36778064,rs759657998 and rs376557691 may be not associated with prevalence of vulnerable carotid plaque.

关 键 词：肌细胞增强因子2A 基因多态性 易损斑块 脑梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学]