松油烯-4-醇对蚕豆蚜虫的毒力及对其Na^+,K^+-ATP酶活力的影响  

Toxicity of terpinen-4-ol to Aphis craccivora Koch(Homoptera:Aphididae)and its effect on activity of Na^+,K^+-ATPase

作　　者：姚陈霞 刘亚飞[1,2] 黄金龙 阮永明 YAO Chenxia;LIU Yafei;HUANG Jinlong;RUAN Yongming(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China;Yunnan Summit Biotechnology Limited Liability Company,Chuxiong 675100,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]云南森美达生物科技有限公司,云南楚雄675100

出　　处：《浙江师范大学学报（自然科学版）》2018年第4期426-430,共5页Journal of Zhejiang Normal University:Natural Sciences

基　　金：浙江省自然科学基金资助项目(LY15C040002);浙江省公益技术研究计划农业项目(LGN18C040001)

摘　　要：为了探索松油烯-4-醇的杀虫毒力及杀虫机理,采用锥形瓶熏蒸法和点滴法,分别测定了松油烯-4-醇对蚕豆蚜虫的熏蒸和触杀毒力,并测定了松油烯-4-醇对蚕豆蚜虫Na+,K+-ATP酶活力的影响.结果表明:松油烯-4-醇对蚕豆蚜虫具有较强的熏蒸和触杀毒力,其LC50和LD50值分别为4. 834 2 mg/L,10. 282 7μg/aphid;松油烯-4-醇对蚜虫的Na^+,K^+-ATP酶具有明显的抑制作用,熏蒸和触杀处理后最高抑制率分别为75. 40%和77. 69%,抑制作用的强度与松油烯-4-醇的剂量存在正相关性.本研究为利用松油烯-4-醇开发成高效无污染的新型植物源农药提供了理论依据.The effects of terpinen-4-ol against aphids fumigation and contact toxicity were investigated.The results showed that terpinen-4-ol had intense fumigant activity and contact toxicity against aphids.The values of LC 50 and LD 50 were 4.834 2 mg/L and 10.282 7μg/aphid respectively.The effect of terpinen-4-ol on Na+,K+-ATPase activity in aphids was investigated in oder to understand the insecticidal mechanism.Terpinen-4-ol had significantly reduced Na+,K+-ATPase activity,the highest inhibition rate were 75.40%and 77.69%after treatment of fumigation and contact.When the dose of terpinen-4-ol increased,the inhibition enhanced.There was a significant correlation between dosage and inhibition.It would provide a theoretical basis for the use of terpinen-4-ol development into efficient pollution-free botanical pesticides.

关 键 词：松油烯-4-醇 蚜虫 熏蒸毒力 触杀毒力 Na+ K+-ATP酶活力 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]