超快速液相色谱-三重四极杆/线性离子阱质谱法同时测定不同贮藏条件下红参中17种人参皂苷类成分  

Simultaneous Determination of 17 Ginsenosides in Different Stored Products of Ginseng Radix et Rhizoma Rubra by UFLC-QTRAP-MS/MS

作　　者：石婧婧 陈舒妤 邹立思[1] 刘训红[1] 唐仁茂[2] 马继梅[2] 严颖[1] 赵慧[1] SHI Jing-jing;CHEN Shu-yu;ZOU Li-si;LIU Xun-hong;TANG Ren-mao;MA Ji-mei;YAN Ying;ZHAO Hui(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Suzhong Yaoye Group Pharmaceutical Limited,Taizhou 225500,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏苏中药业集团股份有限公司,江苏泰州225500

出　　处：《分析测试学报》2018年第11期1294-1301,共8页Journal of Instrumental Analysis

基　　金：国家中药标准化项目(ZYBZH-C-JS-32);江苏高校优势学科建设工程资助项目(YSXK-2014);江苏高校品牌专业建设工程资助项目(PPZY2015A070)

摘　　要：建立了超快速液相色谱-三重四级杆/线性离子阱质谱(UFLC-QTRAP-MS/MS)同时测定红参中17种皂苷成分含量的方法,分析了不同贮藏条件对红参中目标成分的影响。采用SynergiTMHydro-RP 100(100 mm×2. 0 mm,2. 5μm)色谱柱分离,以0. 1%甲酸水-乙腈为流动相,0. 4 m L/min梯度洗脱,在电喷雾负离子(ESI-)模式下,进行多反应监测(MRM)模式检测。根据17种皂苷成分的含量,用聚类分析(HCA)和主成分分析(PCA)对不同贮藏条件红参进行综合评价。结果表明,17种人参皂苷在一定浓度范围内均呈良好的线性关系,相关系数均大于0. 999 0;精密度、重复性和稳定性良好;加标样品的回收率为96. 4%～103%,相对标准偏差(RSD)均小于5%。HCA和PCA结果显示,红参的适宜贮藏条件为以纸箱为外包装避光存放于-20℃。该方法准确、灵敏,为红参药材内在质量的综合评价和全面控制提供了可靠的检测方法,同时为红参适宜贮藏方法的优选提供了基础资料。A comprehensive analytical method based on ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry(UFLC-QTRAP-MS/MS)was developed for the simultaneous determination of 17 ginsenosides in different stored samples of Ginseng Radixet Rhizoma Rubra.Under the optimized chromatographic conditions,good separation for seventeen target compounds were obtained on a Synergi TM Hydro-RP 100 column(100 mm×2.0 mm,2.5μm)using 0.1%aqueous formic acid-acetonitrile as mobile phase at a flow rate of 0.4 mL/min.The target compounds were analyzed under multiple reaction monitoring(MRM)mode with an ESI source operating in negative ion mode,hierarchical cluster analysis(HCA)and principal component analysis(PCA)were used for data processing.The results showed that there existed good linearities for the 17 components in the certain concentration ranges,with their correlation coefficients larger than 0.999 0.The precision,repeatability and stability of the method were all satisfactory.The spiked recoveries for the compounds were between 96.4%and 103%,with relative standard deviations less than 5%.The results showed that the optimum storage conditions for Ginseng Radixet Rhizoma Rubra were as follows:storing it with a carton at-20℃,protected from light.The proposed method is useful for assessment and control on the quality of Ginseng Radix et Rhizoma Rubra,and provides a scientific basis for choosing the most suitable storage condition of Ginseng Radix et Rhizoma Rubra.

关 键 词：红参 超快速液相色谱-三重四极杆/线性离子阱质谱(UFLC-QTRAP-MS/MS) 人参皂苷 贮藏方法 聚类分析 主成分分析 

分 类 号：O657.63[理学—分析化学] TQ460.72[理学—化学]