芥菜型油菜WRKY71转录因子基因家族的鉴定及表达分析  

Identification and Expression Analysis of WRKY71 Transcription Factor Family Genes in Brassica juncea

作　　者：袁玉辉 王舸泓[1] 董浩然 宋博文 刘显军[1] YUAN Yuhui;WANG Kehong;DONG Haoran;SONG Bowen;LIU Xianjun(College of Life Sciences,Resources and Environment Sciences,Key Laboratory of Crop Growth and Development Regulation of Jiangxi Province,Yichun University,Yichun,Jiangxi 336000,China)

机构地区：[1]宜春学院生命科学与资源环境学院,江西省作物生长发育调控重点实验室,江西宜春336000

出　　处：《西北植物学报》2018年第10期1801-1808,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31560404);江西省青年科学基金(20151BAB214016);江西省教育厅科技项目(GJJ161009)。

摘　　要：WRKY转录因子是植物中最大的转录因子家族之一,在植物生长发育及逆境胁迫中起重要作用。该研究在芥菜型油菜基因组概览测序的基础上,采用RT-PCR技术克隆了4个芥菜型油菜WRKY71s基因,分别命名为BjuWRKY71-1-4。生物信息学分析表明,BjuWRKY71-1-4基因开放阅读框(ORF)分别为822、840、834和855bp,分别编码273、279、277和284个氨基酸,预测分子量和等电点分别为30.80、27.11、31.58、32.18kD和7.75、9.08、8.35、7.76。系统进化树分析表明,BjuWRKY71-1-4与白菜WRKY71转录因子相似性最高。亚细胞定位预测分析结果显示,BjuWRKY71s蛋白均定位于细胞核。这4个基因分别定位到芥菜型油菜的A09、B04、A07和scaffold144上。qRT-PCR结果表明,4个BjuWRKY71s基因在高盐、脱落酸和低温胁迫下都有响应。在盐胁迫下,BjuWRKY71-3的表达量呈持续上升趋势,而其他3个基因在6h时表达量达到最大,然后降低;在ABA处理下,BjuWRKY71s基因于6h时表达量最大,随后逐步降低;低温处理后,BjuWRKY71s基因受低温诱导都显著上调表达。BjuWRKY71-4在该研究的3个胁迫条件下响应最为敏感。研究结果为进一步探讨该基因家族对芥菜型油菜逆境调控机制奠定了基础。WRKY transcription factors are one of the largest families of transcriptional regulators in plants,plays an important role in plant growth and development and stress.In this study,based on the result of the genome survey sequencing of Brassica juncea,we cloned four WRKY 71s genes by RT-PCR technology,which named BjuWRKY 71-1-4.Bioinformatics analysis showed that BjuWRKY 71-1-4 contained 822,840,834 and 855 bp ORFs,encoding 273,279,277 and 284 amino acid residues,respectively.It is predicted that the molecular mass of BjuWRKY71-1-4 proteins were 30.80,27.11,31.58,32.18 kD,which isoelectric point were 7.75,9.08,8.35 and 7.76,respectively.Phylogenetic tree analysis showed that BjuWRKY 71-1-4 had the highest similarity with BrWRKY 71 transcription factor.Subcellular localization prediction indicated that BjuWRKY71s were localized to the nucleus.BjuWRKY 71-1-4 genes were located on the chromosome A09,B04,A07 and scaffold144,respectively.Results of qRT-PCR analysis showed that four BjuWRKY 71s genes were responsive to high salt,abscisic acid(ABA)and low temperature stress.Under salt stress,the expression of BjuWRKY 71-3 was increased continuously,while the expression of the other three genes was the highest at 6 h,and then decreased.Under the treatment with ABA,the expression of BjuWRKY 71s gene was the highest at 6 h and then decreased gradually.After low temperature treatment,the expression level of the BjuWRKY 71s was increased significantly.BjuWRKY 71-4 was the most responsive to the abiotic stresses.These results could provide a foundation on further implore the significantly.These results could provide a foundation on further explore the function of BjuWRKY 71s in B.juncea tolerance to abiotic stress.

关 键 词：芥菜型油菜 WRKY71转录因子 非生物胁迫 表达分析 

分 类 号：Q786[生物学—分子生物学] Q789