玉米ZmNPR1基因的克隆及表达分析  

Cloning and Expression Analysis of Maize ZmNPR1 Gene

作　　者：王新涛 杨青 代资举 郝俊杰[2] 王艳 张莹莹 李保全[1] WANG Xintao;YANG Qing;DAI Ziju;HAO Junjie;WANG Yan;ZHANG Yingying;LI Baoquan(Crop Designing Center,He nan Academy of Agricultural Sciences,Zhengzhou 450002,China;Plant Protection Institute,He nan Academy of Agricultural Sciences,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院作物设计中心,郑州450002 [2]河南省农业科学院植物保护研究所,郑州450002

出　　处：《西北植物学报》2018年第10期1817-1822,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：河南省重大科技专项(161100110500);河南省农业科学院科研发展专项(2018YQ30;ynk20177504;2060503)

摘　　要：该研究从玉米高抗自交系D863F中克隆了ZmNPR1基因(GenBank登录号为MH619241)的gDNA和cDNA序列,开放阅读框长1 866bp,编码621个氨基酸,相对分子质量67.61kD,等电点为5.46。系统进化树比对表明,玉米ZmNPR1蛋白和高粱SbNPR1蛋白的亲缘关系较近,相似性高达97%。实时荧光定量PCR结果表明,ZmNPR1在叶片中能够被水稻黑条矮缩病毒诱导并显著上调表达。同时ZmNPR1在玉米叶片、茎、根、雄穗、雌穗以及花丝中均有表达,在雌穗和叶片中的表达量较高。研究表明,ZmNPR1可能在玉米粗缩病抗病过程中起着重要作用。The NPR1,a disease-resistant regulator,plays a key role in plant systemic acquired resistance.In our study,we cloned the gDNA and cDNA sequence of ZmNPR1(GenBank accession number is MH619241)from a maize resistant inbred line D863F.It was found to contain a 1 866 bp open reading frame(ORF),which may encode a 621 amino acid polypeptide with a protein molecular weight of about 67.61 kD and a theoretical isoelectric point of 5.46.Phylogenetic analysis showed that ZmNPR1 was closely related to SbNPR1 of sorghum with 97%similarity.Real time quantitative PCR analysis showed that ZmNPR1 expression could be induced by rice black-streaked dwarf virus(RBSDV)and significantly up-regulated in leaves.Meanwhile,ZmNPR1 was expressed in leaves,stems,roots,tassels,ears and silks,and highly expressed in ears and leaves.The results indicated that ZmNPR1 may play an important role in the maize rough dwarf disease(MRDD)resistance.

关 键 词：玉米 ZmNPR1 克隆 水稻黑条矮缩病毒 

分 类 号：Q785[生物学—分子生物学] Q786