2种地衣共生蓝藻的分离鉴定及其形成藻结皮条件的研究  

Study on the Microbiotic Soil Crust Forming Conditions of Two Photobionts Isolated from Lichens

作　　者：古海尼沙.买买提 吉米拉木.加马力 帕孜来提.拜合提 MAIMAITI Guhainisha;JIAMALI Jimilamu;BAIHETI Pazilaiti(College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《西北植物学报》2018年第10期1936-1944,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31360009;311600052)

摘　　要：以2种地衣——地卷(Peltigera rufescens)和平盘软地卷(Peltigera elisabethae)为研究材料,分离培养地衣共生藻,通过形态特征的观察结合rDNA-ITS序列分析确定其分类地位,并对地衣共生藻在试验室模拟条件下形成人工藻结皮的优化条件进行分析,为地衣共生藻资源的开发和生产实际利用提供依据。结果表明:(1)从平盘软地卷和地卷分离的藻株I1b和L2均属于蓝藻,基于ITS序列构建的系统树分析结果显示,I1b与具鞘微鞘藻(Microcoleus vaginatus)的支持率高达100%,属于具鞘微鞘藻;L2与念珠藻(Nostoc sp.)的支持率为93%,属于念珠藻属。(2)试验室模拟荒漠极端环境中形成人工结皮的优化条件为:沙土含水量10%、接种3∶1混合藻(具鞘微鞘藻:念珠藻)、藻接种量10μg/cm2。In order to provide references to the development and practical utilizations of the lichen symbiotic alga,we isolated two photobionts by using lichen species Peltigera rufescens and Peltigera elisabethae as the main research objects and studied the morphological characters,rDNA-ITS sequences as well as the capacities of the photobionts in the formation of the microbiotic soil crust under the laboratory artificial conditions in this research.(1)The I 1b and L 2 photobionts which were isolated from P.elisabethae and P.rufescens respectively belong to cyanobacteria.According to the analysis of phylogenetic tree which was constructed based on ITS sequences,I 1b was identified as Microcoleus vaginatus with 100%bootstrap value,while culture L 2 was identified as Nostoc sp.with 93%bootstrap value.(2)The most suited conditions for the microbiotic soil crust formation under laboratory artificial desert extreme situation were that 10%of water moisture content,3∶1(M.vaginatus:Nostoc sp.)ratio and 10μg/cm 2 inoculation amount of photobionts.

关 键 词：地衣共生藻 地卷 平盘软地卷 rDNA-ITS序列 藻结皮 

分 类 号：Q949.34[生物学—植物学] Q939.96