DPPH-HPLC-QTOF-MS/MS快速筛选和鉴定杜仲黑茶中抗氧化活性成分  

Rapid Screening and Identification of Antioxidants from Duzhong Brick Tea by DPPH-HPLC-QTOF-MS/MS

作　　者：施树云 郭柯柯[2] 彭胜 童超英[2] 彭密军 SHI Shu-yun;GUO Ke-ke;PENG Sheng;TONG Chao-ying;PENG Mi-jun(National&Local United Engineering laboratory of Integrative Utilization Technology of Eucommia ulmoides,Jishou University,Jishou 416000,China;College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China;Guangdong Institute of Analysis Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals,Guangzhou 510070,China)

机构地区：[1]吉首大学杜仲综合利用技术国家地方联合工程实验室,吉首416000 [2]中南大学化学化工学院,长沙410003 [3]广东测试分析研究所广东省化学危害应急检测技术重点实验室,广州510070

出　　处：《天然产物研究与开发》2018年第11期1913-1917,共5页Natural Product Research and Development

基　　金：国家自然科学基金(31660181);杜仲综合利用技术国家地方联合工程实验室开发基金(NLE201601;NLE201602)

摘　　要：建立DPPH-HPLC-QTOF-MS/MS方法快速筛选、鉴定和测定杜仲黑茶中抗氧化活性成分。杜仲黑茶提取物与DPPH自由基反应30 min后,9个抗氧化活性成分的HPLC峰消失或峰面积明显减少,结合QTOF-MS/MS谱图及与标准品对照,确定抗氧化活性成分为:没食子酸,新绿原酸、原儿茶酸、绿原酸、咖啡酸、表没食子儿茶素没食子酸酯、槲皮素-3-O-桑布双糖苷、芦丁和异槲皮苷。并建立HPLC法同时测定9个抗氧化活性成分的含量。所建立的方法无须繁琐的分离纯化过程,可快速、灵敏、准确、高通量筛选复杂体系中的抗氧化活性成分,并为杜仲黑茶的质量控制提供科学依据。DPPH-HPLC-QTOF-MS/MS was developed for rapid screening,identification and determination of antioxidants from Duzhong brick tea.After reaction with DPPH radical,HPLC peaks of nine antioxidants in Duzhong brick tea extract decreased or disappeared.By analysis of QTOF-MS/MS data,and by comparison with standards,their structures were identified as gallic acid,neochlorogenic acid,protocatechuic acid,chlorogenic acid,caffeic acid,(-)-epigallocatechin gallate,quercetin-3-O-sambubioside,rutin,and isoquercitrin.After that,a HPLC method was established for the simultaneous determination of nine antioxidants.The developed method had the potential to be a high throughput tool for rapid,sensitive,and accurate screening of bioactive compounds from complex matrices without tedious purification procedures,and the results could be used for quality evaluation and quality control of Duzhong brick tea.

关 键 词：DPPH-HPLC-QTOF-MS/MS 杜仲黑茶 抗氧化 多酚 

分 类 号：R284[医药卫生—中药学] Q946.8[医药卫生—中医学]