雌二醇对异位子宫内膜腺上皮细胞增殖及BCL6蛋白表达的影响  

Effects of estradiol on proliferation and BCL6 protein expression of ectopic endometrial glandular epithelial cells

作　　者：魏民[1] 徐凌燕[1] 韩婕 徐燕[1] 樊莉莉[1] WEI Min;XU Lingyan;HAN Jie;XU Yan;FAN Lili(Qinghai Provincial People′s Hospital,Xining 810000,China)

机构地区：[1]青海省人民医院,西宁810000

出　　处：《山东医药》2018年第42期13-15,共3页Shandong Medical Journal

摘　　要：目的观察雌二醇(E2)对异位子宫内膜腺上皮细胞增殖及BCL6蛋白表达的影响。方法收集5例卵巢子宫内膜异位囊肿患者的腹腔镜手术切除标本,获取异位子宫内膜腺上皮细胞。将细胞分为干预组和对照组,分别予以10 nmol/L E2和DMEM/F12培养液,MTT法观察24、48、72 h细胞增殖情况(吸光度值);取干预组干预0、24、48、72 h的细胞,Western blotting法检测细胞中的BCL6蛋白。将细胞分为4组,A、B组转染BCL6-siRNA,C、D组转染对照Scrambeld;转染后,A、C组均予以10 nmol/L E2培养72 h,MTT法观察细胞增殖情况(吸光度值)。结果与对照组同时点比较,干扰组作用48、72 h的细胞吸光度值高(P均<0. 05); E2作用0、24、48、72 h时,干扰组细胞BCL6蛋白相对表达量分别为0. 17±0. 03、0. 19±0. 02、0. 57±0. 06、1. 14±0. 12,48、72 h的BCL6蛋白相对表达量高于0 h时(P均<0. 05)。与D组比较,B组72 h和C组48、72 h的吸光度值高(P均<0. 05);与C组比较,A组48、72 h的吸光度值低(P均<0. 05)。结论 E2可能通过上调BCL6蛋白表达促进异位子宫内膜腺上皮细胞增殖,BCL6蛋白可作为治疗子宫内膜异位的新靶标。To investigate the effects of estradiol(E 2)on the proliferation and BCL6 protein expression of ectopic endometrial glandular epithelial cells.Methods The ectopic endometrial glandular epithelial cells were obtained from 5 cases of patients with ovarian endometriosis cysts for laparoscopic resection.The cells were randomly divided into the intervention group and the control group,and given 10 nmol/L E 2 and DMEM/F12 medium,respectively.MTT assay was used to detect the cell proliferation(absorbance value)at 24,48 and 72 h.Western blotting was used to detect the BCL6 protein level of the cells in the intervention group at 0,24,48,and 72 h.The ectopic endometrial glandular epithelial cells were divided into four groups.The cells in the groups A and B were transfected with BCL6-siRNA,and groups C and D with control Scrambeld.After transfection,cells in the groups A and C were cultured with 10 nmol/L E 2 for 72 h,and then the cell proliferation was detected by MTT assay.Results Compared with the control group at the same time,the absorbance value of the interference group was higher than that of the control group(P<0.05).The relative expression of BCL6 protein was 0.17±0.03,0.19±0.02,0.57±0.06,and 1.14±0.12 in the interference group after treatment with E 2 for 0,24,48,and 72 h,respectively.The BCL6 protein levels at 48 and 72 h were significantly higher than that at 0 h(P<0 05).Compared with the group D,group B had a significantly higher absorbance value at 72 h,and group C had a significantly higher absorbance value at 48 and 72 h(P<0.05).Compared with the group C,group A had a significantly lower absorbance value at 48 and 72 h(P<0.05).Conclusion E 2 may promote the proliferation of ectopic endometrial glandular epithelial cells by up-regulating BCL6 expression,which indicates that BCL6 may be a new target for treatment of endometriosis.

关 键 词：雌二醇 BCL6蛋白 转录抑制因子 子宫内膜异位 子宫内膜腺上皮细胞 细胞增殖 

分 类 号：R711.71[医药卫生—妇产科学]