丙泊酚对氧糖剥夺导致PC12细胞损伤的保护机制  

Protective effects mechanism of propofol on OGD caused neuronal PC12 cells injury

作　　者：祁爱花 王爱忠 王莉[2] QI Aihua;WANG Aizhong;WANG Li(Department of Anesthesiology,Shanghai Sixth People′s Hospital East Affiliated to Shanghai University of Medicine&Health Sciences,Shanghai 201306,China;Department of Anesthesiology, Shanghai Jiaotong University Affiliated Sixth People′s Hospital,Shanghai 200233,China)

机构地区：[1]上海健康医学院附属第六人民医院东院麻醉科,201306 [2]上海交通大学附属第六人民医院麻醉科,200233

出　　处：《重庆医学》2018年第33期4223-4225,共3页Chongqing medicine

基　　金：上海健康医学院种子基金项目(SFP-18-22-14-018)

摘　　要：目的研究丙泊酚对氧糖剥夺(OGD)导致神经元样鼠嗜铬细胞瘤(PC12)细胞损伤的影响及机制。方法用神经生长因子(7S-NGF)将PC12细胞分化为神经元样PC12细胞,于37℃、5%CO2+95%N2中孵育1、6、12、24和48h后,用MTT法获得细胞损伤最明显的时间;用1、5、10μmol/L的丙泊酚分别处理OGD损伤的细胞,通过MTT法得出对细胞作用最显著的浓度。细胞分为3组:正常对照组、OGD组和OGD+丙泊酚组,用免疫细胞化学染色和Western blot评估丙泊酚的作用及机制。结果 PC12细胞OGD损伤12h后,观察到约70%的细胞损伤;10μmol/L丙泊酚处理细胞后,细胞活力增加最为显著(P<0.01);OGD+丙泊酚组磷酸化应激活化蛋白激酶(SAPK)/Jun氨基末端激酶(JNK)和c-Jun的表达明显低于OGD组(P<0.05)。结论丙泊酚通过SAPK/JNK-c-Jun信号通路保护OGD损伤的PC12细胞。Objective To investigate the effect and mechanism of propofol on oxygen-glucose deprivation(OGD)caused rat pheochromocytoma cells(PC12)injury.Methods PC12 cells were differentiated into neuron-like PC12 cells by nerve growth factor(7S-NGF)and then incubated in 5%CO 2+95%N 2 for 1,6,12,24 and 48 h.MTT method was used to detect the most obvious time of cell injury.OGD-injured PC12 cells were treated with 1μmol/L,5μmol/L and 10μmol/L propofol respectively,and the propofol concentration which had most significant effect on cells was obtained by using the MTT method.The cells were randomly divided into three groups:normal control group,OGD group and OGD+propofol group.The effect and mechanism of propofol were evaluated by immunocytochemical staining and Western blot.Results After PC12 cells OGD injury for 12 h,the injury of about 70%cells was observed;after 10μmol/L propofol treating the cells,the cellular viability increase was most significantly(P<0.01).The expression of phosphorylated SAPK/JNK and c-Jun in the OGD+propofol group was significantly lower than that in the OGD group(P<0.05).Conclusion Propofol protects OGD injured PC12 cell through SAPK/JNK-c-Jun signal pathway.

关 键 词：PC12细胞 丙泊酚 应激活化蛋白激酶/Jun氨基末端激酶 氧糖剥夺 

分 类 号：R961.1[医药卫生—药理学]