甘蓝抗软腐病离体鉴定方法探究  

Assessment of inoculation method for identifying resistance to soft rot in cabbage

作　　者：马燕勤 李勤菲[1] 司军[1] 宋洪元[1] 任雪松[1] MA Yanqin;LI Qinfei;SI Jun;SONG Hongyuan;REN Xuesong(College of Horticulture and Landscape Architecture,Southwest University,Chongqing Key Laboratory of Olericulture;Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Chongqing 400715,China)

机构地区：[1]西南大学园艺园林学院,重庆市蔬菜学重点实验室,教育部南方山地园艺学重点实验室,重庆400715

出　　处：《植物保护》2018年第6期136-140,共5页Plant Protection

基　　金：国家重点研发计划(2017YFD0101804);重庆市“十三五”社会事业与民生保障科技创新专项(cstc2015shms-ztzx80007,cstc2015shms-ztzx80009,cstc2015shms-ztzx80005);西南大学博士启动基金(SWU115038)。

摘　　要：通过分析不同因素对甘蓝软腐病离体接种后发病效率的影响,建立了一套甘蓝软腐病抗性的快速鉴定方法。结果发现,用浓度为6.4×10~8 cfu/mL的软腐病液体培养基悬浮液,采用针刺法接种苗龄为5～7叶期植株的离体叶片,接种后空气湿度为90%以上是最有利于软腐病发病的方法。利用该方法鉴定27份栽培甘蓝,发现高抗材料3份,抗病材料8份,耐病材料8份,感病材料8份。A multifactor experiment with three duplicates was established to investigate three different inoculation techniques(transaction inoculation,injection and pin prick)by using pathogenic isolates of Pectobacterium carotovorum subsp.carotovorum in China against resistant and susceptible cabbage cultivars under controlled greenhouse conditions.It was found that the method with the pathogen suspended in the liquid NA medium at the density of 6.4×10 8cfu/mL,inoculating plants at the seedling age of 5-7-leaf old by pin prick in the growth chamber with a high moisture of over 90%,was suitable for the pathogen to infect the cabbage leaves.This method was used to identify resistance to soft rot in 27 cabbage cultivars,three of which showed high resistance,eight moderate resistance,eight tolerance and eight susceptibility to soft rot.

关 键 词：甘蓝 软腐病 离体抗性鉴定 

分 类 号：S436.33[农业科学—农业昆虫与害虫防治]