极低频电磁场暴露对SD大鼠肝、肾和脾脏功能及形态的影响  

Effects of extremely low frequency electromagnetic fields exposure on function and morphology of liver,kidney and spleen of SD rats

作　　者：毛勇[1] 刘浩 吴向阳[1] 高秉仁[1] MAO Yong;LIU Hao;WU Xiangyang;GAO Bingren(Department of Cardiac Surgery,Second Hospital,Lanzhou University,Lanzhou 730000,China;Department of Cardiothoracic Surgery,Central Hospital,Kaifeng City,Henan Province,Kaifeng 475000,China)

机构地区：[1]兰州大学第二医院心脏外科,甘肃兰州730000 [2]河南省开封市中心医院心胸外科,河南开封475000

出　　处：《吉林大学学报（医学版）》2018年第6期1120-1123,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81470026);中央高校科研项目资助课题(lzujbky-2015-47)

摘　　要：目的:探讨极低频电磁场(ELF-EMF)暴露对SD大鼠肝、肾和脾等脏器功能及形态的影响,阐明ELF-EMF辐射损伤的机制。方法:20只雄性SD大鼠随机分为暴露组和对照组,每组10只。暴露组大鼠暴露于磁感应强度为0.1T、频率为50Hz的ELF-EMF装置中,每天8h,连续暴露30d;对照组大鼠不进行上述电磁场暴露。暴露30d后,检测2组大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、尿素氮(UN)和肌酐(Cr)水平,HE染色检测2组大鼠肝脏、肾脏和脾脏形态表现。结果:与对照组比较,暴露组大鼠血清ALT、AST、UN和Cr水平明显升高(P<0.05)。暴露组大鼠肝脏中央静脉及肝血窦、肾脏肾小球及间质毛细血管以及脾脏脾血窦均出现扩张充血,且部分肝细胞出现坏死;对照组大鼠肝脏、肾脏和脾脏形态无异常。结论:ELF-EMF (0.1T、50 Hz)暴露影响SD大鼠肝、肾和脾脏功能及结构,其机制可能与ELFEMF暴露引起的氧化应激反应有关联。Objective:To discuss the effects of extremely low frequency electromagnetic fields(ELF-EMF)exposure on the function and morphology of liver,kidney and spleen of the SD rats,and to clarify the mechanism of radiation damage of ELF-EMF.Methods:Twenty male SD rats were randomly divided into exposure group and control group(n=10).The rats in exposure group were exposed in an ELF-EMF device with a magnetic induction of 0.1T and a frequency of 50 Hz;the exposure was conducted for 8 h per day for 30 d.The rats in control group didn’t receive any treatment.After exposure for 30 d,the serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),urea nitrogen(UN),creatinine(Cr)of the rats in two groups were detected.HE staining was used to detect the morphology of the liver,kidney,spleen tissues of the rats in two groups.Results:Compared with control group,the serum levels of ALT,AST,UN,and Cr of the rats in exposure group were increased(P<0.05).In exposure group,the dilatation and congestion were found in the central veios of liver and hepatic sinusoids,glomeruli and interstitial capillaries of kidney,and insinusoids of spleen of the rats;part of the hepatocytes showed necrosis.There was no abnormality morphology in the liver,kidney,and spleen tissues of the rats in control group.Conclusion:Exposure to ELF-EMF(0.1 T,50 Hz)can affect the function and structures of liver,kidney and spleen tissues of the SD rats,and its mechanism may be related to oxidative stress induced by ELF-EMF.

关 键 词：极低频电磁场 大鼠 SD 肝脏 肾脏 脾脏 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]