辽宁沿海鲬mtDNA控制区结构和遗传多样性分析  

Structure and Genetic Diversity of mtDNA D-Loop Sequences of Sand Gurnard Platycephalus sp.1 Inhabiting Liaoning Coast

作　　者：李玉龙[1] 刘修泽[1] 吉光 于旭光[1] 李轶平[1] 付杰[1] 王小林[1] 郭栋[1] 董婧[1] LI Yulong;LIU Xiuze;JI Guang;YU Xuguang;LI Yiping;FU Jie;WANG Xiaolin;GUO Dong;DONG Jing(Liaoning Key Laboratory of Marine Biological Resources and Ecology,Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China)

机构地区：[1]辽宁省海洋水产科学研究院,辽宁省海洋生物资源与生态学重点实验室,辽宁大连116023

出　　处：《水产科学》2018年第6期818-824,共7页Fisheries Science

基　　金：国家重点研发计划项目(2017YFC1404400);国家自然科学基金面上项目(31770458);辽宁省海洋与渔业科研项目(201401).

摘　　要：用PCR扩增直接测序的方法,获得辽宁沿海25尾鲬线粒体DNA控制区基因的876~878bp全序列。通过GenBank中已有的鲬属鱼类控制区同源序列比对及形态特征分析发现,辽宁沿海鲬实为褐斑鲬而非印度鲬。同时,利用高度变异的控制区序列,对辽宁沿海褐斑鲬进行序列多态性分析和遗传多样性分析。结果显示,25尾褐斑鲬间共有22个变异位点,辽宁沿海褐斑鲬群体的遗传多样性处于中等偏低水平,单倍型多样性指数和核苷酸多样性指数分别为0.92±0.04和0.0026±0.0017。核苷酸不配对分析表明,辽宁沿海褐斑鲬群体在19 000~63 000年前经历了群体扩张。对比其他已报道鱼类控制区的结构识别序列,对鲬属鱼类控制区的结构进行分析,识别了终止序列区、中央保守区和保守序列区,并找到DNA复制终止相关的序列ETAS,中央保守区的保守序列CSB-F、CSB-E、CSB-D以及保守序列区的保守序列CSB1、CSB2、CSB3。这一结果可为将来全面了解鲬的种群分化研究及其物种资源保护提供基础资料。A total of 876-878 bp mtDNA D-loop sequences were amplified in 25 samples of sand gurnard Platycephalus sp.collected from coastal waters in Liaoning province by PCR amplification and directly sequenced.The samples were identified as Platycephalus sp.1 instead of Platycephalus indicus based on morphological characters and DNA barcoding with the aid of the homologous sequences retrieved from GenBank,and the genetic diversity was analyzed by variable mtDNA D-loop sequences.Twenty-two loci of variation were found,with H of 0.92±0.04 andπof 0.0026±0.0017,indicating that the variation level was varied from moderate to low.The mismatch distribution of the control region sequences implied that Platycephalus sp.1 in the Liaoning coast had undergone population expansion,possibly before last 19 000-63 000 years.The mtDNA D-loop sequence of the gurnard was found to be separated into three domains,namely,terminal associated sequence domain,central conserved sequence domain and conserved sequence block domain.The extended terminal associated sequence(ETAS),three conserved sequence blocks(CSB-F,CSB-E,and CSB-D)in the central conserved sequence domain and three conserved sequence blocks(CSB1,CSB2,and CSB3)in the conserved sequence block domain were also identified.The findings provide a foundation for further research on population differentiation and resource conservation of this species.

关 键 词：褐斑鲬 线粒体DNA控制区序列 结构 遗传多样性 

分 类 号：Q346.5[生物学—遗传学]