波纹唇鱼雄激素受体基因的克隆与表达分析  

Cloning and Expression Profiles of Androgen Receptor Gene in Humpheaded Wrasse Cheilinus undulatus

作　　者：邹文慧 单鳞茜 韩邦 骆剑[1] 齐鑫 林浩然[1,2] ZOU Wenhui;SHAN Linqian;HAN Bang;LUO Jian;QI Xin;LIN Haoran(State Key Laboratory of Marine Resource Utilization in South China Sea,Key Laboratory of Tropical Biologicical Resources,Ministry of Education,Hainan University,Haikou 570228,China;Key Laboratory for Aquatic Economic Animals,State Key Laboratory of Biocontrol,Institute of Aquatic Economic Animals,Guangdong Province,Sun Yat-Sen University,Guangzhou 510275,China)

机构地区：[1]海南大学海洋学院,南海海洋资源利用国家重点实验室,热带生物资源教育部重点实验室,海南海口570228 [2]中山大学,水生经济动物研究所,广东省水生经济动物良种繁育重点实验室,广东广州510275

出　　处：《水产科学》2018年第6期835-841,共7页Fisheries Science

基　　金：国家自然科学基金资助项目(31660737);国家“863”计划项目(2012AA10A414);南海海洋资源利用国家重点实验室基金资助项目(2018001);海南省重点研发项目(ZDYF2017038).

摘　　要：通过RT-PCR克隆并得到了波纹唇鱼雄激素受体基因的全长,共3514bp,编码755个氨基酸。氨基酸序列分析表明,该序列有3个结构域,即转录激活区、DNA结合区和配体结合区。系统发育分析表明,波纹唇鱼雄激素受体与贝氏隆头鱼、西氏拟隆头鱼和欧洲舌齿鲈的雄激素受体具有较高的相似性,分别为85.0%、82.5%和79.5%。组织表达分析表明,波纹唇鱼雄激素受体基因在波纹唇鱼的前脑、中脑、后脑、垂体、下丘脑、心脏、肝脏、性腺、脾脏、肾脏、前肠、后肠、肌肉中均有表达,表达量有所差异,肾脏中表达量最为丰富,前肠中表达量最少。波纹唇鱼雄激素受体基因的成功克隆与分析,为波纹唇鱼生殖调控的研究提供了理论基础。The full length cDNA sequence of androgen receptor(ar)in humpheaded wrasse Cheilinus undulatus was cloned by RT-PCR and RACE.The cDNA sequence of ar was found to be 3514 bp,encoding 755 amino acids(aa),and amino acid sequence analysis showed that the deduced amino acid sequence of ar contained all three domains characteristics of ars,transcriptional activation domain,DNA-binding domain and ligand-binding domain.Phylogenetic tree indicated that the ar of humpheaded wrasse had high homology of 85.0%with Labrus bergylta,of 82.5%with Pseudolabrus sieboldi and of 79.5%with Dicentrarchus labrax.The ar was expressed in brain area,heart,liver,gonad,spleen,kidney,foregut,hindgut and muscle,with the maximal expression in the kidney,and the minimal in the foregut.The successful cloning and analysis of ar from humpheaded wrasse provide a theoretical for the study of reproductive regulation.

关 键 词：波纹唇鱼 雄激素受体 克隆 组织表达 

分 类 号：S917[农业科学—水产科学]