乌蒙凤鸡生长激素基因多态性及生物信息分析  

Polymorphism and Bioinformatics Analysis of Growth Hormone Gene in Wumeng Crested Chickens

作　　者：陆静[1,2] 骆科印 王现科 张雄 杨红 莫先艇 黄明捷 张勇 LU Jing;LUO Keyin;WANG Xianke;ZHANG Xiong;YANG Hong;MO Xianting;HUANG Mingjie;ZHANG Yong(Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,Guizhou University,Guiyang 550025,China;Guizhou Province Key laboratory of Animal Genetics,Breeding and Reproduction,College of Animal Science,Guizhou University,Guiyang 550025,China;Agricultural Committee of Liupanshui City,Liupanshui 553000,China;Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang 550025,China;Bijie Animal Husbandry Genetic Resources Management Station,Bijie 551700,China)

机构地区：[1]贵州大学,高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵阳550025 [2]贵州大学动物科学学院,贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [3]贵州省六盘水市市农委,六盘水553000 [4]贵州省农业科学院畜牧兽医研究所,贵阳550025 [5]毕节市畜牧遗传资源管理站,毕节551700

出　　处：《中国畜牧兽医》2018年第11期3122-3133,共12页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省农业科技合作项目:乌蒙凤鸡种质资源地方标准制(701338172201);贵州瑶山鸡、乌蒙凤鸡、柴鸡品系选育研究与推广应用((2017)2533-3)。

摘　　要：试验旨在对乌蒙凤鸡生长激素(growth hormone,GH)基因多态性进行研究,并开展生物信息学分析。以乌蒙凤鸡为研究对象,构建DNA池,PCR扩增GH基因所有外显子和部分内含子,采用直接测序法检测GH基因多态性,利用生物信息学软件对GH蛋白二级结构、三级结构及基本性质(理化性质、疏水性、信号肽、跨膜区、卷曲螺旋区及保守结构域)进行分析。结果显示,乌蒙凤鸡GH基因存在8个SNPs,分别是T1453C、A1489G、A1512G、G2152A、G3206A、G3347A、C3452A和C3453G,其中G2152A、C3452A和C3453G只发生在无凤头乌蒙凤鸡中;C3452A、C3453G位于非编码区,T1453C、A1489G、A1512G、G2152A和G3206A位于内含子上,G3347A位于外显子5。突变后GH基因mRNA结构发生变化,自由能提高,稳定性降低。生物信息学分析表明,GH蛋白为分泌蛋白,含有信号肽序列,有1个典型的卷曲螺旋结构和疏水区,保守结构域在10～214位氨基酸之间,为非跨膜蛋白,且不稳定。结果表明,乌蒙凤鸡GH基因具有较高的遗传多样性,可为乌蒙凤鸡的保种选育提供参考。This study was aimed to explore and analyze the polymorphisms of growth hormone(GH)gene in Wumeng crested chickens.Taking Wumeng crested chickens as the research materials,the blood genomic DNA was extracted to construct DNA pool,all exons and partial introns of GH gene were amplified by PCR,the polymorphisms of GH gene was detected by sequencing,the secondary structure,tertiary structure and basic properties(physicochemical properties,hydrophobicity,signal peptide,transmembrane region,coiled-coil region and conserved domain)of GH protein were analyzed using bioinformatics softwares.The results showed that there were 8 SNPs(T1453C,A1489G,A1512G,G2152A,G3206A,G3347A,C3452A and C3453G)of GH gene in Wumeng crested chickens,there were 3 SNPs(G2152A,C3452A and C3453G)only appeared in flathead Wumeng crested chickens.The polymorphic sites were in different regions,C3452A and C3453G were located in the non-coding region,T1453C,A1489G,A1512G,G2152A and G3206A were located in introns region,and G3347A was located in exon 5.After mutation,the mRNA structure of GH gene were changed,which made the minimum free energy increases,and the stability decreases.Bioinformatics analysis result indicated that GH protein was secreted proteins,which had signal peptide sequence,a typical helical coil structure and a hydrophobic region,there was also a conserved domains in 10-214 amino acids.However,it was not a trans-membrane protein,and the stability was low.The results suggested that GH gene in Wumeng crested chickens had high genetic diversity,which could provide a reference for the conservation breeding of Wumeng crested chickens.

关 键 词：乌蒙凤鸡 凤头 GH基因 多态性 生物信息学 

分 类 号：S813.3[农业科学—畜牧学]