毛竹植硅体微观形态及稳定性的扫描电镜初探  

Stability of Phyllostachys edulis phytolith by scanning electron microscopy

作　　者：赵丽华[1] 黄程鹏 王悦悦[1] 黄张婷 ZHAO Lihua;HUANG Chengpeng;WANG Yueyue;HUANG Zhangting(School of Sciences,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学理学院,浙江杭州311300

出　　处：《浙江农林大学学报》2018年第6期1177-1181,共5页Journal of Zhejiang A&F University

基　　金：浙江省科技计划项目(2015C37069;2016C33028)

摘　　要：为了揭示竹子植硅体表面形态和结构,探究植硅体在缓冲液浸泡后形态是否稳定,以毛竹Phyllostachys edulis叶片为研究材料,采用微波消解法、湿灰化法和干灰化法,提取毛竹叶片中的植硅体。对提取的植硅体经镀金膜处理,用扫描电子显微镜(SU-8000 Hitachi)观测。结果表明:毛竹植硅体具有多种形态。微波消解法提取的毛竹植体长为12~14μm,宽为7~10μm,植硅体呈现长鞍形(竹节形),图像细节清晰,植硅体形态完整;湿灰化法和干灰化法提取的毛竹植硅体呈哑铃形,长约为12μm,连接处宽度约为4μm,两端的宽度约8μm,并且用湿灰化法提取的毛竹植硅体还可见微小突起。微波消解法是提取毛竹植硅体比较理想的方法。微波消解法提取的植硅体在p H 10的硼酸缓冲液中浸泡15 d后的微观形态可见:植硅体外表已出现溶蚀坑,表面变粗糙,可见硅质颗粒,呈现为聚集分布。说明植硅体表面已被破坏,呈现不稳定状态。In order to reveal the surface morphology and structure of bamboo plant phytolith and explore the stability of phytolith after strong alkali soaking,the leaves of Phyllostachys edulis were used as the research material to extract the phytolith by using microwave digestion method,wet ashing method and dry ashing method.The extracted phytolith was treated with gold plated film and observed by scanning electron microscope(SU-8000 Hitachi).The results showed that the phytoliths of Ph.edulis had various forms.The length of phytolith extracted by the microwave digestion method was 12-14μm;the width of phytolith was 7-10μm;the phytolith presented a long saddle shape(slub shaped);the image details were clear and the morphology of the Ph.edulis was complete.The phytolith extracted by wet ashing method and dry ashing method were dumbbell shaped with a length of about 12μm.The width of the joint was about 4μm and the width of the ends was about 8μm.The phytolith extracted by wet ashing could also showed tiny protuberances.Microwave digestion was an ideal method to extract Phytolith from Ph.edulis.The micromorphology of the phytolith extracted by microwave digestion method could be seen after soaking in the boric acid buffer solution with a pH value of 10.The surface of the phytolith appeared corrosion pit and became rough;and the silica particles presented aggregation distribution.It indicated that the surface of the phytolith was damaged and unstable.[Ch,2 fig.22 ref.]

关 键 词：植物学 毛竹 植硅体 扫描电子显微镜 稳定性 微观形态 

分 类 号：S718.4[农业科学—林学]