化州柚花挥发油的GC-MS分析及其对肺癌细胞A549增殖、迁移的影响研究  

GC-MS Analysis of Volatile Oil from the Flowers of Citrus grandis and Study on Effects of It on the Proliferation and Migration of Lung Cancer A549 Cells

作　　者：李武国[1] 苏乔[1] 魏洁书[2] 朱烨妍 刘翠婷 李雯雯[1] 赵广银[1] 黄浩机 詹若挺[4] LI Wuguo;SU Qiao;WEI Jieshu;ZHU Yeyan;LIU Cuiting;LI Wenwen;ZHAO Guangyin;HUANG Haoji;ZHAN Ruoting(Animal Experiment Center,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China;Xinhua College,Sun Yat-sen University,Guangzhou 510520,China;Central Laboratory,Southern Medical University,Guangzhou 510515,China;Research Center of Medicinal Plant Resource Science and Engineering,Guangzhou University of TCM/Key Lab of Chinese Medicinal Resource from Lingnan,Ministry of Education,Guangzhou 510006,China)

机构地区：[1]中山大学附属第一医院动物实验中心,广州510080 [2]中山大学新华学院,广州510520 [3]南方医科大学中心实验室,广州510515 [4]广州中医药大学中药资源科学与工程研究中心/岭南中药资源教育部重点实验室,广州510006

出　　处：《中国药房》2018年第22期3093-3097,共5页China Pharmacy

基　　金：广东省科学技术厅科技计划项目(No.2017A030303015)

摘　　要：目的:提取化州柚花挥发油,鉴定其成分,并评价其对肺癌细胞A549增殖、迁移的体外抑制作用。方法:采用水蒸气蒸馏法提取化州柚花挥发油;采用气质联用法(GC-MS)分析其化学组成,以峰面积归一化法计算各组分的相对百分含量;采用MTT法检测其对肺癌细胞A549增殖的抑制作用,计算细胞相对活力和增殖抑制率;采用细胞划痕实验评价其对肺癌细胞A549迁移能力的影响,计算相对迁移面积和迁移抑制率。结果:从100 g化州柚花中提取得到挥发油0.7 g,得率为0.7%。共从中分离得到了46个组分,鉴定了其中35个组分,占总峰面积的96.95%;相对百分含量较高的化合物包括D-柠檬烯(28.43%)、γ-萜品烯(20.74%)、β-月桂烯(11.97%)、芳樟醇(7.41%)等。与空白对照组比较,120 mg/L化州柚花挥发油可显著降低肺癌细胞A549的相对活力(P<0.05),增殖抑制率上升至21.72%;而1、5、10、20、40、60、80、100 mg/L化州柚花挥发油对肺癌细胞A549的相对活力均无显著影响(P>0.05)。作用24、48 h时,60、120 mg/L化州柚花挥发油可显著缩小肺癌细胞A549的相对迁移面积(P<0.05),迁移抑制率分别为132.46%、125.08%(24 h)和124.54%、124.66%(48 h);而30 mg/L化州柚花挥发油对肺癌细胞A549的相对迁移面积均无显著影响(P>0.05)。结论:GC-MS法可用于鉴定化州柚花挥发油的化学组成。化州柚花挥发油以D-柠檬烯、γ-萜品烯、β-月桂烯等成分为主,对肺癌细胞A549的增殖、迁移能力具有一定的体外抑制作用。OBJECTIVE:To extract and identify the volatile oil from the flowers of Citrus grandis,and evaluate inhibitory effects of it on the proliferation and migration of lung cancer A549 cells in vitro.METHODS:The volatile oil was extracted from the flowers of C.grandis by steam distillation.The chemical constituents were analyzed by GC-MS.Relative percentage of each component was calculated by peak area normalization.Inhibitory effect of volatile oil on the proliferation of lung cancer A549 cells was detected by MTT assay,and the relative activity and proliferation inhibitory rate were calculated.Inhibitory effect of volatile oil on the migration of lung cancer A549 cells was evaluated by cell scratch test,and the relative migration area and migration inhibitory rate were calculated.RESULTS:Totally 0.7 g volatile oil was obtained from 100 g flowers of C.grandis with a yield of 0.7%.A total of 46 chemical components were isolated from the volatile oil,among which there were 35 compounds identified,accounting for 96.95%of the total peak area.The compounds with relatively higher percentage content included D-limonene(28.43%),γ-terpinene(20.74%),β-myrcene(11.97%)and linalool(7.41%),etc.Compared with blank control group,120 mg/L volatile oil from the flowers of C.grandis could significantly reduce the relative activity of lung cancer A549 cells(P<0.05),and the proliferation inhibitory rate increased to 21.72%.The volatile oil from the flowers of C.grandis had no obvious inhibitory effects on the relative activity of lung cancer A549 cells at the concentration of 1,5,10,20,40,60,80,100 mg/L(P>0.05).After treated for 24,48 h,the volatile oil from the flowers of C.grandis could significantly reduce the relative migration area of lung cancer A549 cells at the concentration of 60,120 mg/L(P<0.05);and the migration inhibitory rate were 132.46%,125.08%(24 h)and 124.54%,124.66%(48 h),respectively.But 30 mg/L volatile oil from the flowers of C.grandis had no obvious inhibitory effects on the relative migration area of lung cancer A

关 键 词：化州柚花 挥发油 气质联用法 增殖 迁移 肺癌细胞A549 体外抑制作用 

分 类 号：R284.1[医药卫生—中药学] R734.2[医药卫生—中医学]