蒙药复方述达格-4特征图谱初步分析  

Preliminary Analysis of Characteristic Chromatograms of Mongolian Medicine Compound Shudage-4

作　　者：杜银飞 嵇增云 信莎莎 董玉 DU Yinfei;JI Zengyun;XIN Shasha;DONG Yu(College of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学药学院,呼和浩特010110

出　　处：《中国药房》2018年第22期3110-3114,共5页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81260680);内蒙古自治区科技创新引导项目(No.1639003)

摘　　要：目的:分析蒙药复方述达格-4的特征图谱。方法:采用高效液相色谱法。色谱柱为Agilent Extend C18,流动相为0.4%乙酸溶液-甲醇(梯度洗脱),流速为0.6 m L/min,检测波长为286 nm,柱温为30℃,进样量为20μL。分别对复方述达格-4全方及各单味药体内外特征图谱进行分析和对比。结果:复方述达格-4全方体外特征图谱有47个峰;其体内特征图谱有24个峰,其中峰1、4、7～9、12、14、18～20、22、25、38～41、44、46对应的18个成分为体外直接入血成分;各单味药体内特征图谱中峰1、4、7、8、12、14、18～22、25、40、46来源于高良姜药材;峰14、18、19、22、44来源于木香药材;峰1、4、7～9、12、14、18、19、22来源于石菖蒲药材。结论:直接入血成分及代谢产物可能为复方述达格-4在体内发挥作用的有效成分;该方法可用于其特征图谱的分析。OBJECTIVE:To analyse characteristic chromatograms of Mongolian Compound Shudage-4.METHODS:HPLC method was adopted.The determination was performed on Agilent Extend C18 with mobile phase consisted of 0.4%acetic acid solution-methanol(gradient elution)at the flow rate of 0.6 mL/min.The detection wavelength was set at 286 nm,and column temperature was 30℃.The sample size was 20μL.The in vivo and in vitro characteristic chromatograms of allsquare of Compound Shudage-4 and single ingredients were analysed and compared respectively.RESULTS:Totally 47 peaks were identified from in vitro characteristic chromatograms of allsquare of Compound Shudage-4.There were 24 common peaks from in vivo characteristic chromatograms,among which 18 ingredients corresponding to common peaks 1,4,7-9,12,14,18-20,22,25,38-41,44,46 were direct blood components in vitro;peaks 1,4,7,8,12,14,18-22,25,40 and 46 of serum test sample were from Alpinia officinarum;peaks 14,18,19,22,44 were from Aucklandia lappa;peaks 1,4,7-9,12,14,18,19,22 were from Acorus tatarinowii.CONCLUSIONS:Blood components and some metabolites may beallsquare of Compound Shudage-4.The method can be used for the analysis of characteristic chromatograons of it.

关 键 词：蒙药 复方述达格-4 特征图谱 化学成分 体内 体外 

分 类 号：R284.1[医药卫生—中药学]