锦鲤疱疹病毒感染鲤鱼的动态病理损伤及病原组织分布  

Dynamic Distribution of Cyprinid Herpesvirus 3 in Infected Cyprinus carpio and Its Hispathological Changes

作　　者：何汶璐 李根[1] 康慧敏 何嘉乐 杨壮志[2] 阳瑞雪 汪开毓[1] 耿毅[1] 欧阳萍[1] HE Wenlu;LI Gen;KANG Huimin;HE Jiale;YANG Zhuangzhi;YANG Ruixue;WANG Kaiyu;GENG Yi;OUYANG Ping(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China;Institute of Fisheries,Chengdu Academy of Agriculture and Forosttry Sciences,Chengdu 611130,China)

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]成都市农林科学院水产研究所,四川成都611130

出　　处：《云南农业大学学报（自然科学版）》2018年第5期884-890,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：中国博士后科学基金(2015M582563);教育部留学回国人员科研启动基金[教外司留(2015)311号]

摘　　要：【目的】研究锦鲤疱疹病毒(CyHV-3)感染鲤鱼的侵染规律与动态病理损伤,为CyHV-3致病机理的研究奠定实验基础,同时为有效防控锦鲤疱疹病毒病提供参考。【方法】采用浸泡法对鲤鱼进行人工感染试验,于不同时间点剖检采样,进行动态病理学观察和荧光定量PCR检测,选择具有代表性的外部(鳃)和内部器官(肾脏)进行动态病理学观察。【结果】鳃小片呼吸上皮细胞增生严重并伴有大量炎症细胞浸润,肾脏主要表现为间质性肾炎,肾小管上皮细胞变性坏死。试验鱼在感染前期病毒含量最高的是鳃组织,肠道中未检测到病毒,随着感染时间的延长,各组织内的病毒含量逐步增加。病毒感染10 d后,鳃、肾脏以及脑组织的病毒含量达到最高值,随后病毒量保持稳定。脾脏、肝脏和肠道中病毒含量的最高值出现在感染第14天。【结论】CyHV-3在鲤鱼体内先入侵鳃组织,随后到达肾脏、脾脏等全身各组织器官中。[Purpose]To study the infection process and pathological damage of CyHV-3 infected carp,and provide valuable reference for the effective prevention and cure of koi herpesvirus disease.[Method]Carp(Cyprinus carpio)were infected with CyHV-3 and dissected at different time points for detecting the histopathological changes and distribution of virus.And gills and kidney were selected as representative of the external organs and internal organs for dynamic pathological observation.[Result]The gill of test fish respiratory epithelial proliferation and a large number of inflammatory cell infiltration.Besides,kidney tissue was significantly inflammatory cell infiltration,show-ing interstitial nephritis.Then,the gill tissue on the 2nd day of infection was detected by real-time PCR with the largest viral load,and no virus was detected in the intestine.With the infection time extended,the viral load in organizations gradually increased,and the viral load of gill,kidney and brain tissue reached the highest value on the 10th day of virus infection,then it remained stable.In addition,the highest amount of virus in the spleen,liver and intestine was detected on the 14th day of infection.[Conclusion]The gills are the first internal invasion sites of CyHV-3,then the virus reached the kidney,spleen and other tissues and organs.

关 键 词：锦鲤疱疹病毒 鲤鱼 病理损伤 病原组织分布 

分 类 号：S941.419[农业科学—水产养殖]