微生物肥料对草莓根腐病防治效果及对根围土壤微生物群落多样性的影响  

Effect of Microbial Fertilizer on the Control of Strawberry Root Rot and the Diversity of Soil Microbial Communities

作　　者：李丽艳 朱瑞艳[4] 杜迎辉 肖艳 LI Li-yan;ZHU Rui-yan;DU Ying-hui(Leading Biological and Agricultural Limited Company,Qinhuangdao,Hebei 066004;Agricultural Biotechnology Engineering Technology Research Center of Hebei Province,Qinhuangdao,Hebei 066004;Key Laboratory of Plant Nutrition and Marine Functional Fertilizer,Hebei Provincial Department of Agriculture,Qinhuangdao,Hebei 066004;College of Environmental and Chemical Engineering,Yanshan University,Qinhuangdao,Hebei 066004)

机构地区：[1]领先生物农业股份有限公司,河北秦皇岛066004 [2]河北省农业生物技术工程技术研究中心,河北秦皇岛066004 [3]河北省农业厅植物营养与海洋功能肥料重点实验室,河北秦皇岛066004 [4]燕山大学环境与化学工程学院,河北秦皇岛066000

出　　处：《安徽农业科学》2018年第33期111-113,共3页Journal of Anhui Agricultural Sciences

基　　金：河北省科技计划项目(17273201D);河北省产业创新创业团队项目(169A76114H)

摘　　要：[目的]探究微生物施肥技术对草莓根腐病的防治效果,为进一步合理应用微生物肥料防治草莓重茬病害提供理论依据。[方法]在草莓生长期施用微生物肥料,于膨果期调查草莓根腐病发病情况,并采集土样提取土壤基因组DNA,通过PCR扩增建立文库,利用Miseq平台Illumina第二代高通量测序技术并结合相关生物信息学分析土壤细菌16S r RNA基因V3+V4区域和真菌ITS1区域的丰富度和多样性指数以及群落结构。[结果]微生物肥料可使草莓根腐病发病率降低36.3%,平均防效达63.9%;显著降低镰孢菌属的相对丰度,较对照区降低了97.56%,明显提高了有益细菌芽孢菌属的相对丰度,较对照区提高了30.37%。[结论]微生物肥料可有效改善土壤微生物区系并提高作物产量、改善草莓品质。[Objective]To investigate the effect of microbial technology on the control of strawberry root rot,as well as to provide a theoretical basis for the further application of microbial technology to control strawberry stubble.[Method]The microbial fertilizer was applied during the growth of strawberry,and the occurrence of strawberry root rot was investigated in swelling period.The soil genomic DNA was extracted and the gene library was amplified by PCR.Miseq platform Illumina next generation sequencing and the bioinformatics were used to analyze the abundance and diversity indices of soil bacterial 16S rRNA gene V3+V4 region and fungal ITS1 region as well as the community structure.[Result]The incidence of strawberry root rot decreased by 36.3%,the average control efficiency reached 63.9%,and the relative abundance of Fusarium spp.decreased by 97.56%compared with the control.The relative abundance of beneficial bacteria was increased by 30.37%compared with the control.[Conclusion]Microbial fertilizer could improve soil microbial flora and crop yield,and improve the quality of strawberry.

关 键 词：草莓根腐病 微生物肥料 微生物群落多样性 

分 类 号：S144[农业科学—肥料学]