明胶海绵/β-磷酸三钙复合物联合不同剂量碱性成纤维细胞生长因子诱导骨再生的评价  

Effect of gelatin sponge/beta-tricalcium phosphate composite with different doses of basic fibroblast growth factor on bone regeneration

作　　者：崔婷婷 邱泽文 贵林[1] 仲维剑[1] 马国武[1] Cui Tingting;Qiu Zewen;Gui Lin;Zhong Weijian;Ma Guowu(Hospital of Stomatology,Stomatology College of Dalian Medical University,Dalian 116023,Liaoning Province,China;Animal Laboratory Center,Dalian Medical University,Dalian 116044,Liaoning Province,China)

机构地区：[1]大连医科大学口腔医学院附属口腔医院,辽宁省大连市116023 [2]大连医科大学实验动物中心,辽宁省大连市116044

出　　处：《中国组织工程研究》2019年第2期190-195,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省自然科学基金(2015010313-301;项目负责人:仲维剑~~

摘　　要：背景:目前,常用的提高组织再生率的方法是将生长因子与支架材料相结合,因此基因增强的骨组织工程方法越来越受到重视。目的:评价明胶海绵/β-磷酸三钙复合物联合不同剂量碱性成纤维细胞生长因子的骨再生效果。方法:取40只雄性SD大鼠,制备颅骨缺损模型,随机分4组干预,于骨缺损处分别植入单纯明胶海绵/β-磷酸三钙复合物、载10,100,200ng碱性成纤维细胞生长因子的明胶海绵/β-磷酸三钙复合物。植入后8周取植骨材料及其下的颅骨,通过大体和组织学观察分析成骨情况。结果与结论:各组明胶海绵/β-磷酸三钙复合物被大量的纤维结缔组织包裹,纤维结缔组织中可见大量的新生血管及淋巴细胞,在β-磷酸三钙颗粒周围可见巨噬细胞,β-磷酸三钙颗粒未见明显吸收,β-磷酸三钙与颅骨间有新骨形成,新骨骨小梁之间有较多活跃的骨细胞,编织骨结构紊乱,编织骨下方为成熟的板层骨,是未破坏的原有颅骨;载100ng碱性成纤维细胞生长因子的明胶海绵/β-磷酸三钙复合物组新骨形成量最多,单纯明胶海绵/β-磷酸三钙复合物组新骨形成量最少。结果表明,碱性成纤维细胞生长因子联合明胶海绵/β-磷酸三钙复合物可促进骨缺损中的骨再生,其中以100 ng碱性成纤维细胞生长因子促进骨再生效果最好。BACKGROUND:The current method of improving tissue regeneration is to combine growth factors with scaffold materials.Therefore,more and more attention has been paid to the gene-enhanced tissue engineering method.OBJECTIVE:To evaluate the effect of gelatin sponge/β-tricalcium phosphate composite mixed with different doses of basic fibroblast growth factor on bone regeneration.METHODS:Forty male Sprague-Dawley rats were selected to prepare skull defect models.Rat models were randomly divided into four groups,and implanted with gelatin sponge/β-tricalcium phosphate composite,gelatin sponge carrying 10,100 and 200 ng of basic fibroblast growth factor/β-tricalcium phosphate composite.After 8 weeks of implantation,bone formation was observed by gross and histological observations.RESULTS AND CONCLUSION:In each group,the gelatin sponge/β-tricalcium phosphate composite was encapsulated by a large number of fibrous connective tissues,a large number of neovascularization and lymphocytes distributed in the fibrous connective tissue,and macrophages were visible aroundβ-tricalcium phosphate granules.β-tricalcium phosphate granules were not obviously absorbed,and there was new bone formation betweenβ-tricalcium phosphate and the skull.Many active bone cells existed among the new bone trabeculae,with disordered woven bone structure,the mature lamellar bone underneath the woven bone,and the undamaged original skull.The most active new bone formation was found in the basic fibroblast growth factor 100 ng+gelatin sponge/β-tricalcium phosphate composite group,and the new bone volume was the least in the solely used gelatin sponge/β-tricalcium phosphate composite.In conclusion,the combination of basic fibroblast growth factor with gelatin sponge/β-tricalcium phosphate composite can promote bone regeneration in the bone defect,and the best effect on bone regeneration is observed when 100 ng basic fibroblast growth factor is used.

关 键 词：骨再生 bFGF 碱性成纤维细胞生长因子 明胶海绵/β-磷酸三钙复合物 组织工程 动物实验 生物材料 成纤维细胞生长因子2 磷酸钙类 骨再生 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]