干细胞心肌向分化成熟过程中的物理刺激方法  

Physical stimulation methods promote myocardial differentiation and maturation of stem cells

作　　者：张泽茜 伍佳琪 樊瑜波[1,2] 郑丽沙[1,2] Zhang Zeqian;Wu Jiaqi;Fan Yubo;Zheng Lisha(School of Biological Science and Medical Engineering, Beihang University,Beijing 100083,China;Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,Beijing 100083,China)

机构地区：[1]北京航空航天大学生物与医学工程学院生物力学与力生物学教育部重点实验室,北京市100083 [2]北京航空航天大学生物医学工程高精尖创新中心,北京市100083

出　　处：《中国组织工程研究》2019年第1期110-117,共8页Chinese Journal of Tissue Engineering Research

基　　金：科技部国家重点研发计划(2017YFC0108505);项目负责人:郑丽沙;国家自然科学基金(11572030);项目负责人:郑丽沙;国家自然科学基金(11120101001;11421202;11827803);项目负责人:樊瑜波;中央高校基本科研业务费专项经费(111计划)(B13003);项目负责人:郑丽沙~~

摘　　要：背景:寻找提高干细胞心肌向分化成熟度的方法成为心脏疾病治疗研究的热点。目的:总结和分析了提高干细胞心肌向分化成熟度的物理刺激方法及研究新进展。方法:以"干细胞、心肌细胞、机械刺激、电刺激、基质、三维培养、成熟度"为中文关键词,以"stem cell,cardiomyocyte,mechanical stimulation,electrical stimulation,substrate,three-dimensional culture,maturity"为英文关键词,使用计算机检索PubMed、中国期刊全文数据库(CNKI)等数据库,纳入方法规范、逻辑严谨、与物理因素刺激干细胞心肌向分化成熟相关的研究性论文,排除个案研究、方案设计不合理的研究、无规范严谨纳入标准的综述性研究及与论述主题无关的研究。结果与结论:通过检索文献,总结了提高干细胞心肌向分化成熟度的各种物理刺激因素,包括其作用机制及研究进展,为临床使用干细胞移植治疗心脏疾病带来了全新的方法,但目前的研究仍存在一些需要解决的问题,如移植细胞死亡、免疫排斥反应、心律失常和致瘤等,限制了其在临床的应用。BACKGROUND:Methods for improving the myocardial differentiation of stem cells have become a hot topic in the treatment of heart diseases.OBJECTIVE:To summarize and analyze the physical stimulation methods and research advances in improving the myocardial differentiation of stem cells.METHODS:A online computer search of PubMed,Chinese Journals Full-text Database(CNKI)and other literature databases was conducted using the keywords of“stem cell,cardiomyocyte,mechanical stimulation,electrical stimulation,substrate,three-dimensional culture,maturity”in English and Chinese,respectively.Research literature related to normative,logical and rigorous methods as well as physical factors for stimulating myocardium maturation differentiated from stem cells.Case studies,studies with no rational standard design program,review with no rigorous inclusion criteria,and studies having nothing to do with the topic were excluded.RESULTS AND CONCLUSION:Through the literature search,a variety of physical stimuli,mechanisms of action and research progress have been summarized to improve myocardial differentiation and maturity of stem cells.The use of stem cell transplantation for the treatment of heart disease has brought new methods for clinical use,but there are still some problems to be solved,such as transplanted cell death,immune rejection,arrhythmia and tumorigenesis,which have limited the clinical application of stem cells.

关 键 词：干细胞 心肌细胞 机械刺激 电刺激 三维培养 成熟度 国家自然科学基金 干细胞 肌细胞 心脏 物理刺激 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]