硒对邻苯二甲酸酯亚慢性暴露雄性大鼠肝脏的保护作用  

Effects of selenium on livers of male rats with sub-chronic exposure of diethylhexyl phthalate

作　　者：宁俊康 曹乾[1] 杨梅 任雪丹[3] 王民生[3] NING Junkang;CAO Qian;YANG Mei;REN Xuedan;WANG Minsheng(School of Public Health of Southeast University,Nanjing 210009;Wujiang District Center for Disease Control and Prevention, Suzhou 215200;Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,Jiangsu,China)

机构地区：[1]东南大学公共卫生学院,江苏南京210009 [2]苏州市吴江区疾病预防控制中心,江苏苏州215200 [3]江苏省疾病预防控制中心,江苏南京210009

出　　处：《癌变．畸变．突变》2018年第6期479-482,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:研究邻苯二甲酸酯(DEHP)亚慢性暴露对雄性大鼠肝脏的影响及硒对大鼠肝脏的保护作用。方法:将77只雄性大鼠随机分为7组,分别设置为对照组(饲喂基础饲料)、3个DEHP染毒组(染毒剂量分别为300、600和900mg/kg)、3个硒干预组(在相应剂量的染毒组中加入1mg/kg酵母硒)。染毒8周,染毒期末,大鼠空腹16h后称量大鼠体质量、采血进行生化检测以及称量肝脏质量。结果:与对照组相比,600和900mg/kgDEHP染毒组大鼠的平均体质量明显降低(P<0.05);900mg/kgDEHP染毒组大鼠的体质量增量、总摄食量、总食物利用率、明显降低(P<0.01或P<0.05);各DEHP染毒组、硒干预组的肝脏平均质量、肝脏系数均明显降低(P<0.01)。600mg/kgDEHP染毒后的硒干预组大鼠体质量增量较相应的染毒组增加(P<0.05)。生化指标检测结果显示,与对照组相比,600、900mg/kgDEHP染毒组,300、600mg/kgDEHP染毒后的硒干预组大鼠ALT浓度明显升高(P<0.05);900mg/kgDEHP染毒组,300、600和900mg/kgDEHP染毒后的硒干预组大鼠AST浓度明显升高(P<0.01);600、900mg/kgDEHP染毒组大鼠TP浓度明显升高(P<0.01);900mg/kgDEHP染毒组,300mg/kgDEHP染毒后的硒干预组大鼠ALB浓度明显升高(P<0.01);900mg/kgDEHP染毒组大鼠GLB浓度明显升高(P<0.01)。900mg/kgDEHP染毒后的硒干预组大鼠ALT、AST、TP、GLB均明显低于相应的染毒组(P<0.01或P<0.05)。结论:DEHP的亚慢性暴露对雄性大鼠的体质量与生长产生一定的影响,可能造成肝脏肿胀,对肝脏功能产生影响;而硒的干预可能在一定程度上缓解DEHP对肝脏的毒性作用。OBJECTIVE:To investigate effects of sub-chronic exposure to diethylhexyl phthalate(DEHP)on livers of male rats and protective effect of selenium on the livers.METHODS:Seventy-seven male rats were randomly divided into 7 groups:control group(feeding basal feed),3 DEHP-treatment groups(diluted doses of 300,600 and 900 mg/kg,respectively),and 3 Selenium intervention groups(1 mg/kg yeast selenium was added to the corresponding dose of the exposure group).After 8 weeks of exposure,the rats were weighed,blood samples were collected for biochemical detection and the excised liver was weighed after 16 hours fasting.RESULTS:Compared with the control group,the mean weight of livers and coefficient of the liver were significantly increased in each of the exposed groups and the intervention group(P<0.01).In addition,the quality of the 600 mg/kg treated group was significantly reduced(P<0.05);the 900 mg/kg treated group was significantly lower in the body weight gain index(P<0.01),and significantly lower than the control group in the total food intake and utilization index(P<0.05);Compared with the treated group,the Se+300 mg/kg intervention group showed a significant decrease in weight gain(P<0.05).The results of biochemical indicators showed that,compared with the control group,the 600 mg/kg treated group showed a significant increase in TP(P<0.01);the 900 mg/kg treated group showed a significant increase in all five indicators(P<0.01);The Se+300 mg/kg intervention group significantly increased the ALT index(P<0.01),and significantly increased the ALT and AST indexes(P<0.01).The Se+600 mg/kg intervention group significantly increased the ALT index(P<0.05).There was a significant increase in AST index(P<0.01).The Se+900 mg/kg intervention group showed significantly increased AST,TP and GLB(P<0.01).Compared with the treated group,the intervention group was lower than the corresponding exposure group,and the Se+900mg/kg intervention group showed a significant decrease in TP and GLB indicators(P<0.01).CONCLUSION:Sub-chronic exp

关 键 词：邻苯二甲酸酯 大鼠 硒 肝脏 

分 类 号：R994.6[医药卫生—毒理学]