紫花苜蓿MsUGT73B2基因的克隆及表达  

Cloning and Expression of MsUGT73B2 Gene in Medicago sativa

作　　者：尤章 张靖 任鹏辉[1] 杨培志[1] 呼天明[1] YOU Zhang;ZHANG Jing;REN Penghui;YANG Peizhi;HU Tianming(College of Animal Science and Technology,Northwest A&F University,Yangling Shaanxi,712100,China)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农业学报》2018年第10期1508-1517,共10页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家牧草产业技术体系(CARS-34);国家自然科学基金(31772660;31572456)~~

摘　　要：糖基化是植物体蛋白、激素等物质的常见修饰方式,对维持植物正常生长发育具有重要作用,在植物中糖基化反应由糖基转移酶催化。利用蒺藜苜蓿参考序列通过RACE法克隆得到紫花苜蓿基因MsUGT73B2的全长序列,利用生物信息学方法分析其氨基酸序列、二级结构、理化性质等,RT-qPCR分析基因表达情况,构建基因表达载体,研究亚细胞定位。结果表明,MsUGT73B2基因全长1 512bp,编码503个氨基酸,属于稳定的亲水性蛋白,序列比对结果显示其属于UGT家族基因,亚细胞定位于细胞质中。MSOGT73B2主要在紫花苜蓿叶片中表达,且干旱、10μmol/L ABA处理下在2h之前升高,之后下降;在150mmol/L NaCl胁迫处理下表现为4h之前表达水平升高,之后下降的趋势。Glycosylation is a common modification of plant proteins,hormones and other substances,and plays an important role in maintaining the normal growth and development of plants.Glycosylation in plants is catalyzed by glycosyltransferases.The full-length sequence of MsUGT73B2 gene was cloned by RACE-PCR using reference sequence of Medicago truncatula,and its amino acid sequence,secondary structure,and physicochemical properties were analyzed by bioinformatics.The gene expression was analyzed by qRT-PCR.By constructing gene expression vector,we studied gene’s subcellular localization.The result showed that the full-length MsUGT73B2 gene was 1 512 bp in length and encoded 503 amino acids.It was a stable hydrophilic protein.The sequence alignment showed that it belonged to the UGT family.The subcellular localization result showed that the MsUGT73B2 gene played a role in the cytoplasm.The gene was mainly expressed in leaves.The expression of the gene increased in 2 h under drought and 10μmol/L ABA treatments,and then decreased;the expression level of the gene increased in 4 h under 150 mmol/L NaCl treatment,and then decreased.

关 键 词：紫花苜蓿 糖基转移酶 MsUGT73B2 表达模式 

分 类 号：S812.4[农业科学—草业科学]