乳胶附聚型阴离子交换固定相的制备及表征  

Preparation and characterization of a latex-agglomerated anion exchange chromatographic stationary phase

作　　者：孙雨安[1] 王超威 李振兴[1] 于文浩 刘军伟 朱岩[2] SUN Yu’an;WANG Chaowei;LI Zhenxing;YU Wenhao;LIU Junwei;ZHU Yan(Department of Applied Chemistry,Zhengzhou University of Light Industry,Zhengzhou 450000,China;Department of Chemistry,Zhejiang University,Hangzhou 310028,China)

机构地区：[1]郑州轻工业学院应用化学系,河南郑州450000 [2]浙江大学化学系,浙江杭州310028

出　　处：《色谱》2018年第12期1206-1210,共5页Chinese Journal of Chromatography

基　　金：郑州轻工业学院博士基金项目(0112-13501050071)~~

摘　　要：以烯丙基缩水甘油醚(AGE)为乳胶聚合单体,制备了一种乳胶附聚型阴离子交换固定相。通过无皂化乳液聚合法,以AGE和苯乙烯(ST)为共聚单体制备AGE-ST共聚乳胶。将该乳胶季铵化后附聚在磺化的聚苯乙烯-二乙烯苯(PS-DVB)微球表面,制备一种乳胶附聚型阴离子交换色谱固定相。通过扫描电镜(SEM)、傅里叶红外光谱(FTIR)和元素分析(EA)等对该乳胶附聚型阴离子交换色谱固定相的理化性质进行表征,结果显示季铵化的AGE-ST共聚乳胶成功附聚在磺化的PS-DVB微球表面,并通过分离常规阴离子和有机酸对制得的阴离子交换剂的色谱性能进行评价。AGE以其良好的pH耐受性和活泼的反应活性为离子交换色谱固定相的制备提供一个新的选择。Allyl glycidyl ether(AGE)was selected as the copolymerization monomer to prepare a copolymer latex-agglomerated anion exchange chromatography stationary phase for ion chromatography(IC).First,allyl glycidyl ether-styrene(AGE-ST)copolymer latex was prepared by the saponification emulsion polymerization method.It was then quaternized by reacting alternatively with methylamine(MA)and 1,4-butanedioldiglycidyl ether(BDDGE)and agglomerated on the surface of sulfonated polystyrene-divinylbenzene(PS-DVB)microspheres to obtain a copolymer latex-agglomerated anion exchange chromatography stationary phase.The stationary phase was characterized by scanning electron microscopy(SEM),FT-IR and elemental analysis(EA).It was found that the diameters of the PS-DVB microsphere and AGE-ST copolymer latex were about 6μm and 300 nm,respectively.The quaternized AGE-ST copolymer latex was successfully agglomerated on the surface of PS-DVB microspheres and the nitrogen content increased as quaternization time was increased.The chromatographic properties were evaluated by separating conventional anions and organic acids.The results showed that the quaternized AGE-ST copolymer latex-agglomerated PS-DVB anion exchange column exhibited good separation properties toward anions.Therefore,based on the good pH toleration and reaction activity of the newly developed stationary phase,it was concluded that using AGE as a monomer was a good choice.

关 键 词：离子色谱 固定相 季铵化 阴离子交换 乳胶附聚 烯丙基缩水甘油醚 

分 类 号：O658[理学—分析化学]