葛根素减轻七氟烷对人神经胶质瘤细胞H4的损伤  

Puerarin attenuates sevoflurane induced injury in human neuroglioma cell H4

作　　者：朱桂红 甯交琳 鲁开智 ZHU Gui-hong;NING Jiao-lin;LU Kai-zhi(Dept.of Anesthesiology,the First Hospital Affiliated to Army Medical University,Chongqing 400038,China)

机构地区：[1]陆军军医大学第一附属医院手术麻醉科,重庆400038

出　　处：《基础医学与临床》2018年第12期1733-1736,共4页Basic and Clinical Medicine

摘　　要：目的观察葛根素对七氟烷引起的H4神经胶质瘤细胞损伤的保护作用。方法体外培养人H4神经胶质瘤细胞,并分为4组:对照组、七氟烷组(4. 1%七氟烷培养6 h)、葛根素组(仅加入100μmol/L葛根素培养)和葛根素干预组(在接受七氟烷刺激前1 h加入100μmol/L葛根素)。CCK8检测细胞活性; Caspase 3活性试剂盒和流式细胞计量术检测细胞凋亡; DCFH-DA探针检测细胞ROS水平;分光光度法检测细胞中丙二醛(MDA)和超氧化物歧化酶(SOD)含量; Western blot检测淀粉样蛋白前体蛋白裂解酶1(BACE1)表达。结果 4. 1%七氟烷可明显降低H4细胞活性(P<0. 01),增加细胞caspase 3活化和凋亡率(P<0. 01),并增加ROS和MDA的产生及BACE1的表达(P<0. 01),降低SOD水平,而100μmol/L葛根素预处理可明显减轻上述损伤。结论葛根素可通过减少H4细胞凋亡,氧化应激及BACE1的表达产生保护作用,这可能为预防吸入麻醉药导致的阿尔茨海默病提供新的防治策略。Objective To observe the effect of puerarin on the injury of H4 human neuroglioma cells induced by sevoflurane.Methods H4 human neuroglioma cells were cultured in vitro and divided into 4 groups:control group;sevoflurane group(cells were treated with 4.1%sevoflurane for 6 h);puerarin(cells were cultured only with 100μmol/L puerarin for 6 h);sevoflurane+puerarin group(cells were pre-treated with 100μmol/L puerarin for 1 h before the expose to sevoflurane for 6 h).CCK8 assay was used to detect the cell viability.Cell apoptosis was detected by caspase 3 activity kit and flow cytometry.The production of reactive oxygen(ROS)was tested by probe DCFH-DA.Spectrophotometry was used to evaluate the malondialdehyde(MDA)and superoxide dismutase(SOD).Western blot was used to assess expression of BACE1.Results Compared with control group,cell viability of sevoflurane group was significantly decreased(P<0.01),apoptosis and the expression of BACE1 was obviously increased(P<0.01),the level of ROS and MDA was significant increased(P<0.01)while the activities of SOD was significantly depressed(P<0.01).However,the puerarin observably attenuated these changes(P<0.01).Conclusions Puerarin protected H4 human neuroglioma cells against apoptosis and oxidative stress and attenuated the expression of BACE1 induced by sevoflurane,which suggest that it may be a new strategy for prevention of Alzheimer’s disease caused by inhalation anesthetics.

关 键 词：葛根素 七氟烷 阿尔茨海默病 凋亡 氧化应激 

分 类 号：R969.2[医药卫生—药理学]