狂犬病两株不同毒力克隆株病毒的神经毒力和全长基因序列比较  

Comparison of neurovirulence and full-length sequence of two rabies virus clones with different virulence

作　　者：石磊泰[1] 李玉华[1] 俞永新[1] SHI Lei-tai;LI Yu-hua;YU Yong-xin(Arboviral Vaccine Division,National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院虫媒病毒疫苗室,北京102629

出　　处：《中国人兽共患病学报》2018年第11期986-990,共5页Chinese Journal of Zoonoses

摘　　要：目的对2株毒力不同的狂犬病病毒克隆株(CTN181-3和CTN181-12)进行神经毒力和全长基因序列的比较。方法分别对2株病毒用10、14和21日龄小鼠脑内接种,测定小鼠的致死力(LD_(50));同时用空斑法测定2株病毒的病毒滴度(PFU),以lgPFU/lgLD_(50)比较2株病毒的毒力差异;分别对2株病毒进行全基因序列测定并分析,找出毒力差异关键基因位点。结果 2株病毒对10、14和21日龄小鼠的lgPFU/lgLD_(50),CTN181-3株为0.07、3.40和6.60,CTN181-12株为<-0.9、<-0.6和1.04。全基因序列分析表明,二者共有8个核苷酸和6个位点的氨基酸存在差异。结论 CTN181-3株的神经毒力明显低于CTN181-12株,两者间6个氨基酸的差异是其毒力差异的分子基础。We aimed to compare the neurovirulence and genome sequence differences between two rabies virus clones with different virulence.The different ages(10 d,14 d and 21 d)of mice were tested for the neurovirulence(LD 50)of the two clones by intracerebral inoculation.The inoculated virus content(PFU)was tested by plaque assay.The lgPFU/lgLD 50 values of the two clones were calculated and compared.The full-length genomes of the two clones were sequenced and compared.The results showed the values of lgPFU/lgLD 50 tested in the 10 d,14 d,and 21 d-old mice with CTN181-3 strain were higher(0.07,3.40 and 6.60,respectively)than that of the CTN181-12 strain(<-0.9,<-0.6 and 1.04,respectively),which indicated the neurovirulence of CTN181-3 strain was lower than that of CTN181-12 strain.The full-length sequence analysis showed that there were eight nucleotides and six amino acids differences between the two virus clones.The neurovirulence of CTN181-3 is lower than that of CTN181-12 strain and the six amino acids difference between them are the molecular basis for the neurovirulence.

关 键 词：狂犬病减毒株 克隆株 毒力 全基因序列分析 

分 类 号：R373.9[医药卫生—病原生物学]