小鼠泡型包虫囊液对小鼠骨髓来源树突状细胞表达IDO的影响  

Effect of multilocular cyst fluid on IDO expression in mouse bone marrow derived dendritic cells

作　　者：付永[1,2] 孟茹 姜涛[4] 张壮志[5] 王宏宾[6] FU Yong;MENG Ru;JIANG Tao;ZHANG Zhuang-zhi;WANG Hong-bin(Academy of Animal and Veterinary Medicine,Qinghai University,Xining 810016,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China;Animal Husbandry and Veterinary Station of Xining,Xining 810016,China;The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;Veterinary Research Institute,Xinjiang Academy of Animal Sciences,Urumqi 830000,China;The Affiliated Hospital of Qinghai University,Xining 810001,China)

机构地区：[1]青海大学畜牧兽医科学院,西宁810016 [2]青海大学三江源生态与高原农牧业国家重点实验室,西宁810016 [3]西宁市畜牧兽医站,西宁810016 [4]新疆医科大学第一附属医院,乌鲁木齐830054 [5]新疆畜牧科学院兽医研究所,乌鲁木齐830000 [6]青海大学附属医院,西宁810001

出　　处：《中国人兽共患病学报》2018年第11期1001-1005,共5页Chinese Journal of Zoonoses

基　　金：青海省科学技术厅基础研究项目(No.2016-ZJ-760)资助~~

摘　　要：目的探讨小鼠泡型包虫囊液(MCF)诱导小鼠骨髓来源树突状细胞(BMDCs)表达IDO的能力。方法通过体外诱导培养获得BMDCs,分别与MCF(MCF组)、rmIFN-γ(阳性对照组)和RPMI-1640培养基(阴性对照组)进行共培养,在不同时间点收集各组BMDCs,采用Real-time PCR方法检测BMDCs表面IDO mRNA转录水平,Western blotting方法检测BMDCs表面IDO蛋白水平,ELISA方法检测BMDCs上清液中IDO浓度变化。结果 MCF组BMDCs表面IDO mRNA相对表达量和BMDCs上清液中IDO浓度在24h达到峰值,明显高于阴性对照组(F=303.305,5752.473,P<0.01);而MCF组BMDCs表面IDO蛋白相对表达水平在48h达到最高值,明显高于阴性对照组,差异有统计学意义(F=70.971,P<0.01)。结论 MCF具有上调BMDCs表面IDO表达的能力,为进一步揭示泡型包虫病的免疫耐受分子机制提供了理论依据。The objective of this study was to explore the ability of multilocular cyst fluid(MCF)to induce IDO expression in mouse bone marrow derived dendritic cells(BMDCs).BMDCs were obtained by induction culture in vitro,and coculture with MCF(MCF group),rmIFN-gamma(positive control group)and RPMI-1640 culture medium(negative control group).BMDCs of each group were collected at different time points.Real-time PCR method was used to detect IDO mRNA transcriptional level on BMDCs.Western blotting method was used to detect IDO protein level on BMDCs.ELISA method was used to detect the variation of IDO concentration in BMDCs supernatant.The results showed that MCF group of relative expression of IDO mRNA on BMDCs and IDO concentration in BMDCs supernatant reached peak value at 24 h,which were greatly higher than the negative control group(F=303.305,5 752.473,P<0.01).While MCF group of relative expression level of IDO protein on BMDCs reached the highest value at 48 hours,which wer e significantly higher than that in negative control group(F=70.971,P<0.01).These results indicate that MCF has the ability to up regulate the expression of IDO on BMDCs,which provides a theoretical basis for further revealing the molecular mechanism of immune tolerance in alveolar echinococcosis.

关 键 词：泡型包虫病 树突状细胞 吲哚胺2 3-双加氧酶 免疫耐受 

分 类 号：R383.3[医药卫生—医学寄生虫学]